Baetens D, Stefan Y, Ravazzola M, Malaisse-Lagae F, Coleman D L, Orci L
Diabetes. 1978 Jan;27(1):1-7. doi: 10.2337/diab.27.1.1.
Endocrine-cell populations in the islets of Langerhans of mutant mice with a severe hypoinsulinemic diabetes (ob/ob or db/db on the C57BL/KsJ background) or with a mild hyperinsulinemic diabetes (ob/ob or db/db on the C57BL/6J background) were studied quantitatively by immunofluorescence and morphometry. In severely diabetic mice, islets presented a reduced proportion of insulin containing cells but increased glucagon-, somatostatin-, and pancreatic polypeptide (PP)-containing cells, as compared with islets of control (+/+) mice. An inverse change was observed in islets of mildly diabetic mice: islets were hypertrophic and composed mostly of insulin-containing cells, with decreased proportions of glucagon-, somatostatin-, and PP-containing cells. In both types of diabetic syndromes, the changes in cell populations induced a qualitative alteration of cellular interrelationships in the affected islets.
通过免疫荧光和形态测量法对具有严重低胰岛素血症糖尿病（C57BL/KsJ背景的ob/ob或db/db）或轻度高胰岛素血症糖尿病（C57BL/6J背景的ob/ob或db/db）的突变小鼠胰岛中的内分泌细胞群进行了定量研究。在严重糖尿病小鼠中，与对照（+/+）小鼠的胰岛相比，胰岛中含胰岛素细胞的比例降低，但含胰高血糖素、生长抑素和胰多肽（PP）的细胞增加。在轻度糖尿病小鼠的胰岛中观察到相反的变化：胰岛肥大，主要由含胰岛素细胞组成，含胰高血糖素、生长抑素和PP的细胞比例降低。在这两种糖尿病综合征中，细胞群的变化导致受影响胰岛中细胞间关系的质性改变。
