Alanazi Asma, Munir Hafsa, Alassiri Mohammed, Ward Lewis S C, McGettrick Helen M, Nash Gerard B
Institute of Cardiovascular Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK.
College of Medicine, King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia.
Biorheology. 2019;56(1):15-30. doi: 10.3233/BIR-180185.
Mesenchymal stem cells (MSC) are used in therapy, often by injection into the blood.
We aimed to compare the adhesive and migratory properties of MSC from umbilical cords (UCMSC), bone marrow (BMMSC) or trabecular bone (TBMSC), which might influence delivery to injured tissue.
MSC were perfused through glass capillaries coated with matrix proteins, collagen or fibronectin, or albumin. Adherent cells were counted microscopically and their spreading analysed over time. MSC migration through 8 μm pore filters coated with the same proteins was analysed.
The number of MSC adhering to collagen was greater than fibronectin, decreased as wall shear rate increased from 17 to 70 s-1, and was in the order UCMSC>BMMSC>TBMSC. Conversely, spreading was more effective on fibronectin and was in the order BMMSC>TBMSC≥UCMSC. Migration was promoted by coating the lower surface of filters with either matrix protein, with UCMSC migrating more efficiently than BMMSC.
MSC show origin-dependent variations in their efficiency of capture from flow and subsequent spreading or ability to migrate on matrix proteins. UCMSC showed most efficient capture from flow, which was followed by less spreading, but more rapid migration. These responses might be associated with more effective delivery from the circulation into damaged tissue.
间充质干细胞（MSC）常用于治疗，通常通过注射到血液中。
我们旨在比较来自脐带（UCMSC）、骨髓（BMMSC）或小梁骨（TBMSC）的MSC的黏附与迁移特性，这些特性可能会影响其向损伤组织的递送。
将MSC灌注通过涂有基质蛋白、胶原蛋白或纤连蛋白或白蛋白的玻璃毛细管。在显微镜下对贴壁细胞进行计数，并分析其随时间的铺展情况。分析了MSC通过涂有相同蛋白质的8μm孔径滤膜的迁移情况。
黏附于胶原蛋白的MSC数量多于纤连蛋白，随着壁面剪切速率从17 s-1增加到70 s-1而减少，且顺序为UCMSC>BMMSC>TBMSC。相反，在纤连蛋白上的铺展更有效，顺序为BMMSC>TBMSC≥UCMSC。通过用任何一种基质蛋白包被滤膜的下表面可促进迁移，其中UCMSC比BMMSC迁移更有效。
MSC在从血流中捕获并随后铺展的效率或在基质蛋白上迁移的能力方面表现出依赖于来源的差异。UCMSC从血流中捕获的效率最高，随后铺展较少，但迁移较快。这些反应可能与从循环系统向受损组织的更有效递送有关。
