Department of Pathology, Clements University Hospital, University of Texas Southwestern Medical Center, Dallas.
Division of Cardiology, Department of Internal Medicine, Clements University Hospital, University of Texas Southwestern Medical Center, Dallas.
Am J Clin Pathol. 2019 Apr 2;151(5):486-493. doi: 10.1093/ajcp/aqy172.
To investigate biotin interference on three cardiac troponin (cTn) assays and demonstrate a method to overcome biotin interference.
cTn levels were measured in (1) plasma from healthy volunteers on 10-mg daily biotin supplementation mixed with a plasma with known elevated troponin, (2) plasmas with known elevated cTn after mixing in reagent biotin to simulate supplementation, and (3) biotin-spiked plasma specimens pretreated with streptavidin-agarose beads.
Daily biotin ingestion (10 mg) and studies simulating daily biotin use resulted in significant interference in the Gen5 cardiac troponin T (cTnT) assay; the contemporary Gen 4 cTnT and high-sensitivity cardiac troponin I (hs-cTnI) assays were unaffected. The biotin interference threshold was 31, 315, and more than 2,000 ng/mL for Gen5 cTnT, cTnT, and hs-cTnI assays, respectively. Streptavidin pretreatment blocked biotin interference in cTn assays.
Biotin interference is possible at plasma concentrations achievable by ingestion of over-the-counter supplements that may lead to delayed or missed diagnosis of myocardial injury with the Gen5 cTnT assay.
研究生物素对三种心肌肌钙蛋白（cTn）检测试剂的干扰，并提出一种克服生物素干扰的方法。
在（1）每日补充 10mg 生物素的健康志愿者血浆中与已知高肌钙蛋白的血浆混合，（2）在试剂中添加生物素以模拟补充后已知升高的 cTn 的血浆，（3）用链霉亲和素琼脂糖珠预处理的生物素添加血浆标本中测量 cTn 水平。
每日摄入 10mg 生物素和模拟每日使用生物素的研究导致 Gen5 心肌肌钙蛋白 T（cTnT）检测试剂出现显著干扰；而同期的 Gen4 cTnT 和高敏心肌肌钙蛋白 I（hs-cTnI）检测试剂不受影响。Gen5 cTnT、cTnT 和 hs-cTnI 检测试剂的生物素干扰阈值分别为 31、315 和超过 2000ng/mL。链霉亲和素预处理可阻断 cTn 检测试剂中的生物素干扰。
通过摄入非处方补充剂可达到导致 Gen5 cTnT 检测试剂检测心肌损伤出现延迟或漏诊的血浆浓度，这种情况下生物素干扰是可能的。
