Department of Post-Harvest Technology, FRHPHM Division, ICAR-Central Institute of Fisheries Education, Versova, Mumbai, Maharastra, 400061, India.
Fish Nutrition, Biochemistry and Physiology Division, ICAR-Central Institute of Fisheries Education, Versova, Mumbai, Maharashtra, 400061, India.
Environ Sci Pollut Res Int. 2019 Mar;26(9):8875-8884. doi: 10.1007/s11356-019-04144-y. Epub 2019 Feb 4.
Fish protein hydrolysates are digested form of protein with various bioactive properties where, the cleavages of molecular bonds of proteins can be broken by the enzymatic and chemical process. In this study, antioxidant properties of spray dried protein hydrolysate prepared from Pangasius viscera by using enzymatic (papain and pepsin), and chemical methods (hydrochloric acid and sodium hydroxide) were evaluated. Among the different treatments, pepsin-derived visceral protein hydrolysate showed the maximum antioxidant activity when used at higher concentrations. Essential amino acids (EAA) and hydrophobic amino acids are higher in papain-derived visceral protein hydrolysate. In pepsin-derived visceral protein hydrolysate, major proportion was contributed by glycine (Gly), glutamine (Glu), proline (Pro), and asparagine (Asp). Higher amount of aromatic amino acids are found in alkali-derived FVPH. Scanning electron microscopy (SEM) images of pepsin fish visceral protein hydrolysate showed better globular structure than the other treatments. It can be concluded that among the different treatments, the visceral protein hydrolysate prepared with pepsin had better overall quality regarding antioxidant properties and papain in nutritional point of view.
鱼蛋白水解物是一种具有多种生物活性特性的蛋白质消化形式，其中蛋白质的分子键可以通过酶和化学过程断裂。在这项研究中，评估了用酶（木瓜蛋白酶和胃蛋白酶）和化学方法（盐酸和氢氧化钠）从 Pangasius 内脏制备的喷雾干燥蛋白水解物的抗氧化特性。在不同的处理中，当使用较高浓度时，胃蛋白酶衍生的内脏蛋白水解物表现出最大的抗氧化活性。必需氨基酸（EAA）和疏水性氨基酸在木瓜蛋白酶衍生的内脏蛋白水解物中含量更高。在胃蛋白酶衍生的内脏蛋白水解物中，甘氨酸（Gly）、谷氨酸（Glu）、脯氨酸（Pro）和天冬酰胺（Asp）的比例较高。碱衍生的 FVPH 中发现了更多的芳香族氨基酸。胃蛋白酶鱼内脏蛋白水解物的扫描电子显微镜（SEM）图像显示出比其他处理更好的球形结构。可以得出结论，在不同的处理中，用胃蛋白酶制备的内脏蛋白水解物在抗氧化特性和营养方面的整体质量优于其他处理。
