Ramezanpour Mahnaz, Bolt Harrison, Psaltis Alkis, Wormald Peter-John, Vreugde Sarah
Department of Surgery, Otorhinolaryngology Head and Neck Surgery, Queen Elizabeth Hospital, and the University of Adelaide, Adelaide, South Australia, Australia.
Curr Protoc Toxicol. 2019 Jun;80(1):e69. doi: 10.1002/cptx.69. Epub 2019 Feb 4.
Here we use the toll-like receptor (TLR) 3 agonist poly I:C (LMW) to induce an inflammatory response in cells of submerged and/or air-liquid interface (ALI) cultures of human nasal epithelial cells (HNECs). The inflammatory response is determined by measuring interleukin-6 (IL-6) protein levels by enzyme-linked immunosorbent assay (ELISA). The mucosal barrier integrity is determined by measuring transepithelial electrical resistance (TEER) and passage of fluorescently labeled dextrans. Stimulation with poly (I:C) LMW induces a 15- to 17-fold increase in IL-6 production by HNEC-ALI cells. © 2019 by John Wiley & Sons, Inc.
在此，我们使用Toll样受体（TLR）3激动剂聚肌苷酸-聚胞苷酸（LMW）在人鼻上皮细胞（HNECs）的浸没培养和/或气液界面（ALI）培养的细胞中诱导炎症反应。通过酶联免疫吸附测定（ELISA）测量白细胞介素-6（IL-6）蛋白水平来确定炎症反应。通过测量跨上皮电阻（TEER）和荧光标记葡聚糖的通透性来确定黏膜屏障的完整性。用聚（I:C）LMW刺激可使HNEC-ALI细胞产生的IL-6增加15至17倍。©2019约翰威立国际出版公司。
