The College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, PR China.
The College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, PR China.
Int J Biol Macromol. 2019 May 1;128:870-876. doi: 10.1016/j.ijbiomac.2019.01.212. Epub 2019 Feb 1.
In this study, cysteamine-functionalized molybdenum disulfide quantum dots (MoS QDs) were synthesized by a one-pot hydrothermal method. A range of techniques including of Thioflavin T and 8-Anilino-1-naphthalenesulfonic acid fluorescence assays, circular dichroism, and transmission electron microscope have been employed to determination the efficacy of MoS QDs on the inhibition/reversion of fibrillation and hindering cytotoxicity induced by protofibrils and amyloid fibrils of bovine serum albumin (BSA). Results demonstrated that MoS QDs could effectively inhibit the fibrillogenesis and destabilize preformed fibrils of BSA in a concentration-dependent manner. Cytotoxicity protection and imagine on Hela cells was investigated using the methyl thiazolyl tetrazolium (MTT) assay. It was found that MoS QDs not only has good biocompatibility, low toxicity and good cell penetration, but also could effectively decrease the cytotoxicity caused by the formed fibrils of BSA. The results obtained in this work suggested the potential biological application of MoS QDs in therapeutics and provided new insight into the design of multifunctional nanomaterials for amyloid-related diseases.
在这项研究中，通过一锅水热法合成了半胱胺功能化的二硫化钼量子点（MoS QDs）。采用了一系列技术，包括硫代黄素 T 和 8-苯胺-1-萘磺酸荧光测定法、圆二色性和透射电子显微镜，以确定 MoS QDs 对牛血清白蛋白（BSA）原纤维和淀粉样纤维的抑制/逆转纤维形成和抑制细胞毒性的效果。结果表明，MoS QDs 可以有效地抑制纤维形成，并以浓度依赖的方式使 BSA 的预形成纤维不稳定。使用甲基噻唑基四唑（MTT）测定法研究了对 Hela 细胞的细胞毒性保护和成像。结果发现，MoS QDs 不仅具有良好的生物相容性、低毒性和良好的细胞穿透性，而且可以有效降低由 BSA 形成的纤维引起的细胞毒性。这项工作的结果表明，MoS QDs 在治疗学中有潜在的生物学应用，并为设计用于与淀粉样蛋白相关疾病的多功能纳米材料提供了新的思路。
