Unité de Chimie Environnementale et Interactions sur le Vivant EA4492, SFR Condorcet FR CNRS 3417, Université du Littoral Côte d'Opale, 59140, Dunkerque, France.
Centre Commun de Mesures (CCM), Université du Littoral Côte d'Opale, 59140, Dunkerque, France.
Toxicol Lett. 2019 May 1;305:94-102. doi: 10.1016/j.toxlet.2019.01.016. Epub 2019 Feb 1.
Exposure to airborne particulate matter (PM) has significant effects on human health mainly leading to cardio-respiratory diseases. However very few data are available regarding the impact of PM on the skin, so to better understand the impact of fine particle (PM) on both inflammatory response and epidermal structure, we exposed a reconstructed human epidermis (RHE) to several doses of PM collected in Cotonou (Benin, West Africa). After 24 h of exposure, inflammatory response, histological observations, and gene expression related to oxidative stress, antioxidant defense and structural damages were determined. No PM-linked changes in tissue morphology or membrane integrity were observable. PM was however cytotoxic in a dose dependent manner. An inflammatory response appeared as shown by the increase in IL-1α and IL-8 cytokine productions. PM also induced oxidative stress, leading to an increase in 4-HNE immunostaining and to the up-regulation of HMOX1, MT1G and MT1E. Finally, PM had a negative impact on fundamental skin functions such as tissue anchorage, cell differentiation, cornification / skin desquamation and apoptosis. Our data show that airborne fine particles have an adverse effect on skin integrity, most probably leading to accelerated ageing.
暴露于空气中的颗粒物（PM）对人体健康有显著影响，主要导致心肺疾病。然而，关于 PM 对皮肤的影响的数据非常有限，因此，为了更好地了解细颗粒物（PM）对炎症反应和表皮结构的影响，我们将几种剂量的 PM 暴露于在科托努（贝宁，西非）收集的重建人体表皮（RHE）。暴露 24 小时后，测定与氧化应激、抗氧化防御和结构损伤相关的炎症反应、组织学观察和基因表达。没有观察到 PM 与组织形态或膜完整性变化相关的变化。然而，PM 以剂量依赖的方式具有细胞毒性。炎症反应表现为细胞因子 IL-1α 和 IL-8 的产生增加。PM 还诱导氧化应激，导致 4-HNE 免疫染色增加和 HMOX1、MT1G 和 MT1E 的上调。最后，PM 对皮肤的基本功能产生负面影响，如组织锚定、细胞分化、角化/皮肤脱皮和细胞凋亡。我们的数据表明，空气中的细颗粒物对皮肤完整性有不良影响，很可能导致皮肤加速老化。
