Rosa Josyandra Paula de Freitas, Haddad Raphael Federicci, Maeda Fabiana Garcia Reis, Souto Ricardo Peres, Fernandes Cesar Eduardo, Oliveira Emerson de
Faculdade de Medicina do ABC, Santo André, SP, Brazil.
Rev Bras Ginecol Obstet. 2019 Jan;41(1):31-36. doi: 10.1055/s-0038-1676599. Epub 2019 Feb 4.
To evaluate the rs42524 polymorphism of the procollagen type I alpha (α) 2 () gene as a factor related to the development of pelvic organ prolapse (POP) in Brazilian women.
The present study involved 112 women with POP stages III and IV (case group) and 180 women with POP stages zero and I (control group). Other clinical data were obtained by interviewing the patients about their medical history, and blood was also collected from the volunteers for the extraction of genomic DNA. The promoter region of the gene containing the rs42524 polymorphism was amplified, and the discrimination between the G and C variants was performed by digestion of the polymerase chain reaction (PCR) products with the MspA1I enzyme followed by agarose gel electrophoresis analysis.
A total of 292 women were analyzed. In the case group, 71 had the G/G genotype, 33 had the G/C genotype, and 7 had the C/C genotype. In turn, the ratio in the control group was 117 G/G, 51 G/C, and 11 C/C. There were no significant differences between the groups.
Our data did not show an association between the polymorphism and the occurrence of POP.
评估I型前胶原α2（α）基因的rs42524多态性作为巴西女性盆腔器官脱垂（POP）发生相关因素的情况。
本研究纳入112例III期和IV期POP女性（病例组）以及180例0期和I期POP女性（对照组）。通过询问患者病史获取其他临床数据，并采集志愿者血液以提取基因组DNA。扩增包含rs42524多态性的该基因启动子区域，用MspA1I酶消化聚合酶链反应（PCR）产物，随后进行琼脂糖凝胶电泳分析，以区分G和C变体。
共分析292例女性。病例组中，71例为G/G基因型，33例为G/C基因型，7例为C/C基因型。对照组中，相应比例分别为117例G/G、51例G/C和11例C/C。两组间无显著差异。
我们的数据未显示该多态性与POP发生之间存在关联。
