Gomes Lahyre Izaete S, Douhan Greg W, Bibiano Líllian B J, Maffia Luiz A, Mizubuti Eduardo S G
Departamento de Fitopatologia, Universidade Federal de Viçosa, 36570-000 Viçosa, MG, Brazil.
Department of Plant Pathology and Microbiology, University of California, Riverside, CA 92521.
Plant Dis. 2013 Dec;97(12):1537-1543. doi: 10.1094/PDIS-12-12-1212-RE.
A thorough assessment of the distribution of Mycosphaerella spp. associated with banana in Minas Gerais State, Brazil, was conducted after Mycosphaerella fijiensis was first reported to occur in this region in 2005. From 2009 to 2011, 80 fields located in 20 municipalities including the same fields where the disease was first reported were sampled. A total of 800 samples of leaf tissue with symptoms similar to those of yellow or black Sigatoka diseases were examined, and 239 isolates were obtained. The identification of the fungi was based on morphological characters combined with DNA sequences obtained after amplification with species-specific primers and phylogeny inferred from the internal transcribed spacer region of Mycosphaerella strains from banana. All 239 isolates were identified as Mycosphaerella musicola. The absence of M. fijiensis in the samples may have been due to misidentification of M. fijiensis or the displacement of M. fijiensis by M. musicola. It is now apparent that yellow Sigatoka caused by M. musicola is the prevailing leaf spot disease of bananas in Minas Gerais State and that regulatory/legislative control measures need to be revised based on our findings.
在2005年首次报道香蕉黑条叶斑病菌（Mycosphaerella fijiensis）在巴西米纳斯吉拉斯州出现后，对该州与香蕉相关的小球腔菌属（Mycosphaerella spp.）分布情况进行了全面评估。2009年至2011年，在包括该病首次报道地点在内的20个市的80个田地进行了采样。共检查了800份有类似黄叶斑病或黑叶斑病症状的叶片组织样本，获得了239个分离株。真菌的鉴定基于形态特征，并结合用种特异性引物扩增后获得的DNA序列以及从香蕉小球腔菌菌株的内转录间隔区推断出的系统发育。所有239个分离株均被鉴定为香蕉黄条叶斑病菌（Mycosphaerella musicola）。样本中未出现香蕉黑条叶斑病菌可能是由于对该病菌的误鉴定，或者是香蕉黄条叶斑病菌取代了香蕉黑条叶斑病菌。现在很明显，由香蕉黄条叶斑病菌引起的黄叶斑病是米纳斯吉拉斯州香蕉上普遍存在的叶斑病，需要根据我们的研究结果修订监管/立法控制措施。
