CBS Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands;
Persoonia. 2008 Jun;20:19-37. doi: 10.3767/003158508X302212. Epub 2008 Mar 21.
Three species of Mycosphaerella, namely M. eumusae, M. fijiensis, and M. musicola are involved in the Sigatoka disease complex of bananas. Besides these three primary pathogens, several additional species of Mycosphaerella or their anamorphs have been described from Musa. However, very little is known about these taxa, and for the majority of these species no culture or DNA is available for study. In the present study, we collected a global set of Mycosphaerella strains from banana, and compared them by means of morphology and a multi-gene nucleotide sequence data set. The phylogeny inferred from the ITS region and the combined data set containing partial gene sequences of the actin gene, the small subunit mitochondrial ribosomal DNA and the histone H3 gene revealed a rich diversity of Mycosphaerella species on Musa. Integration of morphological and molecular data sets confirmed more than 20 species of Mycosphaerella (incl. anamorphs) to occur on banana. This study reconfirmed the previously described presence of Cercospora apii, M. citri and M. thailandica, and also identified Mycosphaerella communis, M. lateralis and Passalora loranthi on this host. Moreover, eight new species identified from Musa are described, namely Dissoconium musae, Mycosphaerella mozambica, Pseudocercospora assamensis, P. indonesiana, P. longispora, Stenella musae, S. musicola, and S. queenslandica.
三种真菌，即 Mycosphaerella eumusae、M. fijiensis 和 M. musicola，参与了香蕉的 Sigatoka 病复合体。除了这三种主要病原体外，还从 Musa 中描述了几种其他的 Mycosphaerella 或其无性型。然而，这些分类群知之甚少，而且对于这些物种中的大多数，没有培养物或 DNA 可供研究。在本研究中，我们从香蕉中收集了一组全球 Mycosphaerella 菌株，并通过形态学和多基因核苷酸序列数据集进行了比较。从 ITS 区推断的系统发育和包含肌动蛋白基因、小亚基线粒体核糖体 DNA 和组蛋白 H3 基因部分序列的组合数据集揭示了 Musa 上 Mycosphaerella 物种的丰富多样性。形态学和分子数据集的整合证实了香蕉上存在 20 多种 Mycosphaerella（包括无性型）。本研究再次证实了先前描述的 Cercospora apii、M. citri 和 M. thailandica 的存在，并且还在该宿主上鉴定了 Mycosphaerella communis、M. lateralis 和 Passalora loranthi。此外，从 Musa 中鉴定出的八种新物种被描述，即 Dissoconium musae、Mycosphaerella mozambica、Pseudocercospora assamensis、P. indonesiana、P. longispora、stenella musae、S. musicola 和 S. queenslandica。
