College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China.
College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China.
Food Res Int. 2019 Feb;116:518-526. doi: 10.1016/j.foodres.2018.08.068. Epub 2018 Aug 23.
The aim of this study was to isolate and identify antioxidant peptides from defatted walnut meal proteins hydrolysates (DWMPH) prepared by simulated gastrointestinal digestion, and to evaluate the protective effect of the selected antioxidant peptides against oxidative damage to SH-SY5Y cells. The DWMPH were purified by ultrafiltration, gel filtration chromatography and RP-HPLC successively, and finally six major peptides were identified as VRN, NPAN, AHSVGP, SSE, TY and SGGY by UPLC-QTOF-MS. TY and SGGY displayed stronger ABTS radical scavenging activity and higher ORAC value in comparison with other four peptides. Furthermore, 0.5 mg/mL SGGY exhibited significantly protective effect on SH-SY5Y cells against oxidative damage (p < .01). Therefore, it was suggested that walnut meal protein could serve as a potential source of antioxidative peptides through gastrointestinal digestion.
本研究旨在从经模拟胃肠道消化制备的脱脂核桃粕蛋白水解物（DWMPH）中分离和鉴定抗氧化肽，并评价所选抗氧化肽对 SH-SY5Y 细胞氧化损伤的保护作用。DWMPH 经超滤、凝胶过滤色谱和 RP-HPLC 依次纯化，最后通过 UPLC-QTOF-MS 鉴定出 6 种主要肽段，分别为 VRN、NPAN、AHSVGP、SSE、TY 和 SGGY。与其他 4 种肽相比，TY 和 SGGY 显示出更强的 ABTS 自由基清除活性和更高的 ORAC 值。此外，0.5mg/mL 的 SGGY 对 SH-SY5Y 细胞的氧化损伤具有显著的保护作用（p<0.01）。因此，建议通过胃肠道消化，将核桃粕蛋白作为抗氧化肽的潜在来源。
