Wang Jinping, Liu Junmei, John Afiya, Jiang Yueming, Zhu Hong, Yang Bao, Wen Lingrong
College of Food Science and Engineering, Jilin Agricultural University, Changchun, Jilin 130118, China; Guangdong Provincial Key Laboratory of Applied Botany, Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.
College of Food Science and Engineering, Jilin Agricultural University, Changchun, Jilin 130118, China.
Food Chem. 2022 Sep 15;388:132943. doi: 10.1016/j.foodchem.2022.132943. Epub 2022 Apr 11.
Walnut peptides are widely accepted as nutraceuticals with multiple health benefits. However, information about the structure and biological activity of walnut peptides is limited. In this work, walnut protein hydrolysate (WPH) was prepared by enzymatic hydrolysis. The total and free amino acids compositions in WPH were determined. The peptides sequence of WPH was analyzed by UPLC-MS/MS, and forty eight bioactive peptides were identified. Antioxidant activities were detected for WPH in both extracellular and intercellular assays. QGRPWG, PSRADIY and AYNIPVNIAR were the peptides that accounted for the antioxidant activity of WPH. Among them, QGRPWG showed the highest ORAC value (2801 µmol TE/g), and AYNIPVNIAR possessed the strongest protective effect against HO-induced oxidative stress in PC-12 cells in a dose-dependent manner. The results can aid in the recognition of peptides from walnut as nutraceuticals.
核桃肽作为具有多种健康益处的营养保健品被广泛接受。然而，关于核桃肽的结构和生物活性的信息有限。在这项研究中，通过酶解制备了核桃蛋白水解物（WPH）。测定了WPH中的总氨基酸和游离氨基酸组成。通过超高效液相色谱-串联质谱（UPLC-MS/MS）分析了WPH的肽序列，并鉴定出48种生物活性肽。在细胞外和细胞内试验中检测了WPH的抗氧化活性。QGRPWG、PSRADIY和AYNIPVNIAR是导致WPH具有抗氧化活性的肽。其中，QGRPWG的氧自由基吸收能力（ORAC）值最高（2801 μmol TE/g），AYNIPVNIAR对过氧化氢（HO）诱导的PC-12细胞氧化应激具有最强的剂量依赖性保护作用。这些结果有助于将核桃中的肽识别为营养保健品。
