CICS - UBI - Health Sciences Research Centre, University of Beira Interior, 6201-506 Covilhã, Portugal.
CICS - UBI - Health Sciences Research Centre, University of Beira Interior, 6201-506 Covilhã, Portugal.
Food Res Int. 2019 Feb;116:600-610. doi: 10.1016/j.foodres.2018.08.079. Epub 2018 Sep 2.
The aim of the present work was to evaluate the phenolic profile of leaves, stems and flowers of P. avium and their biological potential. For this purpose, two extracts of each matrix (hydroethanolic and infusion) were prepared. A total of twenty-six phenolics were identified by LC-DAD, including 1 hydroxybenzoic acid, 9 hydroxycinnamic acids, 7 flavonols, 3 isoflavones, 3 flavanones and 3 flavan-3-ols, being the hydroethanolic leaves extract the richest one. 5-O-caffeoylquinic acid, hydroxycinnamic derivative 1 and sakuranetin derivative were the major compounds found in leaves, flowers and stems, respectively. The hydroethanolic extracts of stems and leaves proved to be the most active against DPPH and O (IC = 22.37 ± 0.29 μg/mL and IC = 9.11 ± 0.16 μg/mL, respectively). On the other hand, the infusion extract of stems showed the highest antioxidant activity against NO (IC = 99.99 ± 1.89 μg/mL). The antidiabetic potential was tested using the α-glucosidase enzyme, being the infusion extract of stems the most active, with an IC = 3.18 ± 0.23 μg/mL. Finally, the protective effect of the extracts towards human erythrocytes against oxidative damage was also evaluated. The hydroethanolic extract of stems was the most active against lipid peroxidation and hemolysis with an IC = 26.20 ± 0.38 μg/mL and IC = 1.58 ± 0.18 μg/mL, respectively. On the other hand, the hydroethanolic extract of flowers showed the greater protective effect against hemoglobin oxidation (IC = 12.85 ± 0.61 μg/mL). Considering the results obtained in this work, we can consider that leaves, stems and flowers of P. avium are a promising source of bioactive compounds and present health-promoting properties.
本研究旨在评估欧洲甜樱桃的叶、茎和花的酚类成分及其生物活性。为此，从每个基质（水醇提物和浸提液）中制备了两种提取物。通过 LC-DAD 共鉴定出 26 种酚类化合物，包括 1 种羟基苯甲酸、9 种羟基肉桂酸、7 种黄酮醇、3 种异黄酮、3 种黄烷酮和 3 种黄烷-3-醇，其中水醇提物的叶提取物含量最丰富。在叶、花和茎中分别发现 5-O-咖啡酰奎宁酸、羟基肉桂酸衍生物 1 和樱花素衍生物为主要化合物。水醇提物的茎和叶提取物对 DPPH 和 O 的抑制活性最强（IC = 22.37 ± 0.29 μg/mL 和 IC = 9.11 ± 0.16 μg/mL）。另一方面，茎的浸提物对 NO 的抗氧化活性最高（IC = 99.99 ± 1.89 μg/mL）。通过α-葡萄糖苷酶酶测试了其抗糖尿病潜力，结果表明茎的浸提物最具活性，IC = 3.18 ± 0.23 μg/mL。最后，还评估了提取物对人红细胞免受氧化损伤的保护作用。茎的水醇提物对脂质过氧化和溶血的抑制活性最强，IC = 26.20 ± 0.38 μg/mL 和 IC = 1.58 ± 0.18 μg/mL。另一方面，花的水醇提物对血红蛋白氧化的保护作用最强（IC = 12.85 ± 0.61 μg/mL）。考虑到本研究的结果，我们可以认为欧洲甜樱桃的叶、茎和花是生物活性化合物的有前途的来源，并具有促进健康的特性。
