CICS-UBI-Health Sciences Research Centre, University of Beira Interior, 6201-506 Covilhã, Portugal.
UDI-IPG, Research Unit for Inland Development, Polytechnic Institute of Guarda, 6300-749 Guarda, Portugal.
Nutrients. 2018 Nov 5;10(11):1688. doi: 10.3390/nu10111688.
This study aimed to compare three different extracts of sweet cherry, namely the non-colored fraction, colored fraction, and total extract concerning phenolic composition, antioxidant and antidiabetic potential, and erythrocytes' protection and effects on Caco-2 cells. Twenty-two phenolic compounds were identified using high-performance liquid chromatography with diode-array detection. Hydroxycinnamic acids were the most predominant in both the non-colored fraction and total extract, while cyanidin-3--rutinoside was the main anthocyanin found in the colored fraction. The total extract was the most effective against 1,1-diphenyl-2-picrylhydrazyl, nitric oxide, and superoxide radicals, and in the inhibition of α-glucosidase enzyme. The colored fraction revealed the best activity against hemoglobin oxidation and hemolysis. Regarding to Caco-2 cells, the colored extract exhibited the highest cytotoxic effects, while the total extract was the most efficient in protecting these cells against oxidative damage induced by -butyl hydroperoxide.
本研究旨在比较三种不同的甜樱桃提取物，即非色素部分、色素部分和总提取物，比较它们在酚类成分、抗氧化和抗糖尿病潜力、红细胞保护以及对 Caco-2 细胞的影响方面的差异。使用高效液相色谱-二极管阵列检测法鉴定了 22 种酚类化合物。在非色素部分和总提取物中，羟基肉桂酸是最主要的成分，而在色素部分中，矢车菊素-3-O-芸香糖苷是主要的花青素。总提取物对 1,1-二苯基-2-苦基肼基、一氧化氮和超氧自由基的清除作用以及α-葡萄糖苷酶的抑制作用最强。色素部分对血红蛋白氧化和溶血的抑制作用最好。关于 Caco-2 细胞，色素提取物表现出最高的细胞毒性作用，而总提取物在保护这些细胞免受 -丁基过氧化物诱导的氧化损伤方面最为有效。
