Departamento de Ciência e Tecnologia Agroindustrial, Faculdade de Agronomia Eliseu Maciel, Universidade Federal de Pelotas (UFPel), Pelotas, RS, Brazil.
Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas (UFPel), Pelotas, RS, Brazil.
Food Res Int. 2019 Feb;116:652-659. doi: 10.1016/j.foodres.2018.08.092. Epub 2018 Sep 3.
Salmonellosis, caused by the consumption of contaminated foods, is a major health problem worldwide. The aims of this study were to assess the susceptibility of Salmonella spp. isolates to benzalkonium chloride (BC) disinfectant and the antimicrobial activity of Butia odorata Barb. Rodr. extract against the same isolates from food and food environments. Moreover, phenotypic and genotypic resistance profiles, the presence of virulence genes and biofilm forming ability were determined. The minimum inhibitory concentration (MIC) of B. odorata extract against Salmonella spp. ranged from 10 to >19 mg.mL. Resistance to ampicillin, streptomycin, nalidixic acid, sulfonamide, trimethoprim/sulfamethoxazole, trimethoprim, tetracycline, and chloramphenicol was observed. In addition, multidrug resistance was observed in seven isolates (26.92%). The MIC of BC ranged from 32 to 64 mg.L, higher concentrations in comparison with wild-type MICs, and therefore were considered tolerant. Several resistance genes were detected, of which the most common were aadA, qacEΔ1, bla, int1, sul1, and tetA. All isolates carried at least one virulence gene and produced biofilms on stainless steel surfaces at 10 and 22 °C. On the other hand, the B. odorata extract showed activity against Salmonella spp., and it has the potential to be used as a natural antimicrobial to control this important foodborne pathogen, despite its virulence potential and antimicrobial resistance profile.
食源性疾病是全球范围内的一个主要健康问题，由食用受污染的食物引起。本研究旨在评估食品和食品环境中分离的沙门氏菌对苯扎氯铵（BC）消毒剂的敏感性，以及对芽蕉的抗菌活性。此外，还确定了表型和基因型耐药谱、毒力基因的存在和生物膜形成能力。芽蕉提取物对沙门氏菌的最小抑菌浓度（MIC）范围为 10-＞19mg.mL。对氨苄西林、链霉素、萘啶酸、磺胺类药物、甲氧苄啶/磺胺甲恶唑、甲氧苄啶、四环素和氯霉素的耐药性观察到。此外，在 7 株分离株中观察到多药耐药性（26.92%）。BC 的 MIC 范围为 32-64mg.L，与野生型 MIC 相比，浓度更高，因此被认为是耐受的。检测到多个耐药基因，其中最常见的是 aadA、qacEΔ1、bla、int1、sul1 和 tetA。所有分离株均携带至少一种毒力基因，并在 10 和 22°C 时在不锈钢表面形成生物膜。另一方面，芽蕉提取物对沙门氏菌具有活性，尽管其具有潜在的毒力和抗菌耐药性，但它有可能被用作控制这种重要食源性病原体的天然抗菌剂。
