Guangdong Litchi Engineering Research Center, College of Horticulture, South China Agricultural University, Guangzhou 510642, China.
Guangdong Academy of Forestry, Guangzhou 510520, China.
Genes (Basel). 2019 Feb 1;10(2):111. doi: 10.3390/genes10020111.
Warm winters and hot springs may promote panicle leaf growing and repress floral development. To identify genes potentially involved in litchi panicle leaf senescence, eight RNA-sequencing (RNA-Seq) libraries of the senescing panicle leaves under low temperature (LT) conditions and the developing panicle leaves under high temperature (HT) conditions were constructed. For each library, 4.78⁻8.99 × 10⁶ clean reads were generated. Digital expression of the genes was compared between the senescing and developing panicle leaves. A total of 6477 upregulated differentially expressed genes (DEGs) (from developing leaves to senescing leaves), and 6318 downregulated DEGs were identified, 158 abscisic acid (ABA)-, 68 ethylene-, 107 indole-3-acetic acid (IAA)-, 27 gibberellic acid (GA)-, 68 cytokinin (CTK)-, 37 salicylic acid (SA)-, and 23 brassinolide (BR)-related DEGs. Confirmation of the RNA-Seq data by quantitative real-time PCR (qRT-PCR) analysis suggested that expression trends of the 10 candidate genes using qRT-PCR were similar to those revealed by RNA-Seq, and a significantly positive correlation between the obtained data from qRT-PCR and RNA-Seq were found, indicating the reliability of our RNA-Seq data. The present studies provided potential genes for the future molecular breeding of new cultivars that can induce panicle leaf senescence and reduce floral abortion under warm climates.
温暖的冬季和温泉可能会促进穗状叶片的生长，抑制花的发育。为了鉴定可能参与荔枝穗状叶片衰老的基因，构建了低温（LT）条件下衰老穗状叶片和高温（HT）条件下发育穗状叶片的 8 个 RNA 测序（RNA-Seq）文库。每个文库生成 4.78⁻8.99×10⁶ 个清洁读数。在衰老和发育的穗状叶片之间比较了基因的数字表达。总共鉴定出 6477 个上调差异表达基因（DEGs）（从发育叶片到衰老叶片）和 6318 个下调 DEGs，其中 158 个脱落酸（ABA）、68 个乙烯、107 个吲哚-3-乙酸（IAA）、27 个赤霉素（GA）、68 个细胞分裂素（CTK）、37 个水杨酸（SA）和 23 个油菜素内酯（BR）相关 DEGs。通过定量实时 PCR（qRT-PCR）分析对 RNA-Seq 数据进行验证，结果表明，10 个候选基因的 qRT-PCR 表达趋势与 RNA-Seq 揭示的表达趋势相似，并且从 qRT-PCR 和 RNA-Seq 获得的数据之间存在显著的正相关，表明我们的 RNA-Seq 数据是可靠的。本研究为未来的分子育种提供了潜在的基因，这些基因可以在温暖的气候下诱导穗状叶片衰老，减少花的败育。
