Yun Yeojun, Chang Yoosoo, Kim Han-Na, Ryu Seungho, Kwon Min-Jung, Cho Yong Kyun, Kim Hyung-Lae, Cheong Hae Suk, Joo Eun-Jeong
Gwanghwamun Medical Study Centre, Syntekabio Inc., Seoul 03186, Korea.
Department of Occupational and Environmental Medicine, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul 04514, Korea.
J Clin Med. 2019 Feb 2;8(2):173. doi: 10.3390/jcm8020173.
The changes in the gut microbiota of healthy hepatitis B virus (HBV) carriers, including asymptomatic and non-cirrhotic subjects, have been rarely scrutinized. From 1463 faecal samples in health examinees, in total 112 subjects, including 36 hepatitis B surface antigen (HBsAg)-positive and 76 control subjects, were included. Twenty-eight of 36 HBsAg-positive individuals (78%) showed normal alanine aminotransferase (ALT) levels (normal ALT group), whereas eight subjects exhibited elevated ALT levels (22%, high ALT group). By using 16S rRNA gene sequencing, the distance between normal and high ALT groups among HBsAg-positive subjects showed a significant separation after the pairwise comparison of weighted UniFrac distance (permutational analysis of variance -value = 0.039), when compared with the distances to the control group. In comparison with the control group, the normal ALT group had as a significant taxon that showed a positive association (Coefficient () = 0.028, = 0.039). ( = 0.54, = 0.014) and ( = 1.41, = 0.030) showed positive correlations, and ( = -1.31, = 4.15 × 10) exhibited a negative correlation with high ALT level. Gut microbial composition was different according to HBV-induced serum ALT levels, indicative of a potential link between gut and liver metabolism.
健康乙肝病毒（HBV）携带者，包括无症状和非肝硬化患者，其肠道微生物群的变化很少受到仔细研究。在1463份健康体检者的粪便样本中，共纳入了112名受试者，其中包括36名乙肝表面抗原（HBsAg）阳性者和76名对照受试者。36名HBsAg阳性个体中有28名（78%）丙氨酸转氨酶（ALT）水平正常（正常ALT组），而8名受试者ALT水平升高（22%，高ALT组）。通过16S rRNA基因测序，与对照组的距离相比，HBsAg阳性受试者中正常ALT组和高ALT组之间的加权UniFrac距离经成对比较后显示出显著分离（方差置换分析 -值 = 0.039）。与对照组相比，正常ALT组有 作为一个显著的分类群呈现正相关（系数（） = 0.028， = 0.039）。 （ = 0.54， = 0.014）和 （ = 1.41， = 0.030）与高ALT水平呈正相关，而 （ = -1.31， = 4.15 × 10）与高ALT水平呈负相关。肠道微生物组成因HBV诱导的血清ALT水平而异，表明肠道与肝脏代谢之间存在潜在联系。
