Ling A E, Doraisingham S
Department of Pathology, Ministry of Health, Singapore.
Pathology. 1988 Oct;20(4):346-8. doi: 10.3109/00313028809085216.
The efficacy of isolation of influenza viruses was compared in two systems--conventional tube cultures of Madin Darby canine kidney cells, and shell-vial cultures after low-speed centrifugation. One hundred and fifty eight throat and nasal swabs were inoculated and cultures screened by hemagglutination on days 1, 2, 3 and 5 post-inoculation. Twenty-four shell-vial cultures (15.2%) and 6 tube cultures (3.8%) were positive for influenza virus type B, indicating that shell-vial cultures with low-speed centrifugation are superior to tube cultures for influenza virus type B isolation (p less than 0.005 by McNemar's test for analysis of matched pairs data).
在两种系统中比较了流感病毒的分离效果——Madin Darby犬肾细胞的传统试管培养以及低速离心后的转瓶培养。接种了158份咽喉和鼻拭子，并在接种后第1、2、3和5天通过血凝试验对培养物进行筛查。24份转瓶培养物（15.2%）和6份试管培养物（3.8%）B型流感病毒呈阳性，表明低速离心的转瓶培养在分离B型流感病毒方面优于试管培养（通过McNemar检验分析配对数据，p<0.005）。
