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通过空斑试验培养从鼻咽抽吸物中分离甲型流感病毒时，对马-达二氏犬肾细胞（MDCK）与绿猴连续细胞系（Vero）及人肺胚细胞（MRC-5）的比较。

Comparison of Madin-Darby canine kidney cells (MDCK) with a green monkey continuous cell line (Vero) and human lung embryonated cells (MRC-5) in the isolation of influenza A virus from nasopharyngeal aspirates by shell vial culture.

作者信息

Reina J, Fernandez-Baca V, Blanco I, Munar M

机构信息

Virology Unit, Clinical Microbiology Service, University Hospital Son Dureta (UIB), Palma de Mallorca, Spain.

出版信息

J Clin Microbiol. 1997 Jul;35(7):1900-1. doi: 10.1128/jcm.35.7.1900-1901.1997.

DOI:10.1128/jcm.35.7.1900-1901.1997
PMID:9196221
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC229869/
Abstract

We report a comparative study of the MDCK, Vero, and MRC-5 cell lines in the isolation of the influenza A (IA) virus. We studied 746 samples in which 63 IA viruses were isolated. The MDCK line displayed 100% sensitivity, the Vero line displayed 71.4%, and the MRC-5 displayed 57.1%. The MDCK line showed a statistically significant difference with respect to the Vero line (P = 0.001) and the MRC-5 line (P = 0.001). The quantitative sensitivity analysis showed the MDCK line to be superior to the other lines. It seems that the MDCK line is still one of the most recommendable for the isolation of the IA virus from respiratory samples.

摘要

我们报告了一项在分离甲型流感（IA）病毒时对MDCK、Vero和MRC - 5细胞系的比较研究。我们研究了746份样本，从中分离出63株IA病毒。MDCK细胞系显示出100%的敏感性，Vero细胞系为71.4%，MRC - 5细胞系为57.1%。MDCK细胞系与Vero细胞系（P = 0.001）和MRC - 5细胞系（P = 0.001）相比显示出统计学上的显著差异。定量敏感性分析表明MDCK细胞系优于其他细胞系。看来MDCK细胞系仍是从呼吸道样本中分离IA病毒最值得推荐的细胞系之一。

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本文引用的文献

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Evaluation of a direct immunofluorescence assay, dot-blot enzyme immunoassay, and shell vial culture in the diagnosis of lower respiratory tract infections caused by influenza A virus.
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Replication of influenza A viruses in a green monkey kidney continuous cell line (Vero).甲型流感病毒在绿猴肾连续细胞系（Vero细胞）中的复制。
J Infect Dis. 1995 Jul;172(1):250-3. doi: 10.1093/infdis/172.1.250.
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Isolation of influenza virus in human lung embryonated fibroblast cells (MRC-5) from clinical samples.从临床样本中在人肺胚胎成纤维细胞（MRC - 5）中分离流感病毒。
J Clin Microbiol. 1995 Jul;33(7):1948-9. doi: 10.1128/jcm.33.7.1948-1949.1995.
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Rapid viral diagnosis of acute respiratory infections: comparison of enzyme-linked immunosorbent assay and the immunofluorescence technique for detection of viral antigens in nasopharyngeal secretions.急性呼吸道感染的快速病毒诊断：酶联免疫吸附测定法与免疫荧光技术检测鼻咽分泌物中病毒抗原的比较
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Detection of influenza virus by centrifugal inoculation of MDCK cells and staining with monoclonal antibodies.通过对MDCK细胞进行离心接种并用单克隆抗体染色来检测流感病毒。
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6
Successful use of shell vial centrifugation and 16 to 18-hour immunofluorescent staining for the detection of influenza A and B in clinical specimens.成功运用空斑离心法及16至18小时免疫荧光染色法检测临床标本中的甲型和乙型流感。
Am J Clin Pathol. 1989 Oct;92(4):487-90. doi: 10.1093/ajcp/92.4.487.
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Enhancing effect of centrifugation on isolation of influenza virus from clinical specimens.离心对从临床标本中分离流感病毒的增强作用。
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Rapid diagnosis of influenza A infection by direct immunofluorescence of nasopharyngeal aspirates in adults.通过成人鼻咽抽吸物直接免疫荧光法快速诊断甲型流感感染
J Clin Microbiol. 1979 Jun;9(6):688-92. doi: 10.1128/jcm.9.6.688-692.1979.
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Comparison of different tissue cultures for isolation and quantitation of influenza and parainfluenza viruses.用于流感病毒和副流感病毒分离及定量的不同组织培养方法的比较
J Clin Microbiol. 1979 Jul;10(1):32-6. doi: 10.1128/jcm.10.1.32-36.1979.
10
Canine kidney cell line for isolation of respiratory viruses.用于分离呼吸道病毒的犬肾细胞系。
J Clin Microbiol. 1979 Feb;9(2):175-9. doi: 10.1128/jcm.9.2.175-179.1979.