通过空斑试验培养从鼻咽抽吸物中分离甲型流感病毒时，对马-达二氏犬肾细胞（MDCK）与绿猴连续细胞系（Vero）及人肺胚细胞（MRC-5）的比较。
Comparison of Madin-Darby canine kidney cells (MDCK) with a green monkey continuous cell line (Vero) and human lung embryonated cells (MRC-5) in the isolation of influenza A virus from nasopharyngeal aspirates by shell vial culture.
作者信息
Reina J, Fernandez-Baca V, Blanco I, Munar M
机构信息
Virology Unit, Clinical Microbiology Service, University Hospital Son Dureta (UIB), Palma de Mallorca, Spain.
出版信息
J Clin Microbiol. 1997 Jul;35(7):1900-1. doi: 10.1128/jcm.35.7.1900-1901.1997.
Abstract
We report a comparative study of the MDCK, Vero, and MRC-5 cell lines in the isolation of the influenza A (IA) virus. We studied 746 samples in which 63 IA viruses were isolated. The MDCK line displayed 100% sensitivity, the Vero line displayed 71.4%, and the MRC-5 displayed 57.1%. The MDCK line showed a statistically significant difference with respect to the Vero line (P = 0.001) and the MRC-5 line (P = 0.001). The quantitative sensitivity analysis showed the MDCK line to be superior to the other lines. It seems that the MDCK line is still one of the most recommendable for the isolation of the IA virus from respiratory samples.
摘要
我们报告了一项在分离甲型流感（IA）病毒时对MDCK、Vero和MRC - 5细胞系的比较研究。我们研究了746份样本，从中分离出63株IA病毒。MDCK细胞系显示出100%的敏感性，Vero细胞系为71.4%，MRC - 5细胞系为57.1%。MDCK细胞系与Vero细胞系（P = 0.001）和MRC - 5细胞系（P = 0.001）相比显示出统计学上的显著差异。定量敏感性分析表明MDCK细胞系优于其他细胞系。看来MDCK细胞系仍是从呼吸道样本中分离IA病毒最值得推荐的细胞系之一。
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