Mohan Vathsala, Habib Ihab
Institute of Veterinary, Animal and Biomedical Sciences, Massey University, Palmerston North, New Zealand.
School of Veterinary Medicine, Murdoch University, Perth, Western Australia, Australia.
BMC Res Notes. 2019 Feb 4;12(1):76. doi: 10.1186/s13104-019-4107-5.
Campylobacter is a major cause of gastroenteritis in humans and pet ownership is a risk factor for infection. To study the occurrence, species distribution and sequence-based types of Campylobacter spp. in pet cats, 82 faecal samples were collected from cats in New Zealand. The PCR positive samples of Campylobacter jejuni were characterized by multilocus sequence typing (MLST), major outer membrane protein gene (porA) and flagellin A gene (flaA) sequence typing.
Seven faecal samples were tested positive for Campylobacter spp. (9%, or 4-17% at 95% confidence interval), of which six were identified as C. jejuni, and one was C. upsaliensis. The six C. jejuni isolates were characterised by MLST; four belonged to ST-45 clonal complex and two of the isolates could not be typed. Two flaA-SVR types were identified: three samples were flaA-SVR type 8 and one belonged to 239. By combining all data, three isolates were indistinguishable with allelic combinations of ST-45, flaA-SVR 8, porA 44, although no epidemiological connection between these isolates could be established. To conclude, healthy cats can carry C. jejuni, whose detected genetic diversity is limited. The isolated sequence type ST-45 is frequently reported in human illnesses.
弯曲杆菌是人类肠胃炎的主要病因，饲养宠物是感染的一个风险因素。为研究宠物猫中弯曲杆菌属的发生情况、物种分布及基于序列的类型，从新西兰的猫中采集了82份粪便样本。对空肠弯曲菌的PCR阳性样本进行多位点序列分型（MLST）、主要外膜蛋白基因（porA）和鞭毛蛋白A基因（flaA）序列分型。
7份粪便样本弯曲杆菌属检测呈阳性（9%，95%置信区间为4 - 17%），其中6份鉴定为空肠弯曲菌，1份为乌普萨拉弯曲菌。对6株空肠弯曲菌分离株进行MLST分型；4株属于ST - 45克隆复合体，2株无法分型。鉴定出两种flaA - SVR类型：3份样本为flaA - SVR 8型，1份属于239型。综合所有数据，3株分离株在ST - 45、flaA - SVR 8、porA 44的等位基因组合上无法区分，尽管这些分离株之间无法建立流行病学联系。总之，健康猫可携带空肠弯曲菌，其检测到的遗传多样性有限。分离出的序列类型ST - 45在人类疾病中经常被报道。
