USDA, ARS, National Animal Disease Center, Ames, IA, United States.
USDA, ARS, National Laboratory for Agriculture and the Environment, Ames, IA, United States.
Vaccine. 2019 Feb 28;37(10):1255-1259. doi: 10.1016/j.vaccine.2018.12.058. Epub 2019 Feb 1.
Salmonella vaccine strategies for food-producing animals have typically focused on a specific serovar that either causes production losses due to morbidity/mortality or is an important food safety pathogen for a particular food commodity. The Salmonella enterica serovar Typhimurium BBS 866 vaccine strain was designed to reduce serovar specificity to provide cross-protection against diverse Salmonella serovars, thereby broadening its applicability for multiple animal and poultry species. We reported cross-protection of the BBS 866 vaccine in swine [Vaccine 34:1241-6]. In the current study, we extend the efficacy of the Salmonella vaccine to a poultry commodity by revealing significant reduction of multidrug-resistant Salmonella enterica serovar Heidelberg colonization of the cecum and systemic dissemination to the spleen in BBS 866-vaccinated turkeys. Transcriptional analysis of whole blood from BBS 866-vaccinated turkeys revealed down-regulation of metabolic and immune genes (KCNAB1, ACOD1, GPR17, ADOR2AB, and IL-17RD), suggesting limited leukocyte activation without an overt peripheral inflammatory response to vaccination.
用于食用动物的沙门氏菌疫苗策略通常侧重于特定的血清型，这些血清型要么因发病/死亡率而导致生产损失，要么是特定食品商品的重要食品安全病原体。肠沙门氏菌血清型 Typhimurium BBS 866 疫苗株旨在降低血清型特异性，提供针对多种沙门氏菌血清型的交叉保护，从而扩大其在多种动物和家禽中的适用性。我们报道了 BBS 866 疫苗在猪中的交叉保护作用[疫苗 34:1241-6]。在当前的研究中，我们通过揭示 BBS 866 疫苗接种可显著减少多药耐药性沙门氏菌血清型海德堡定植于盲肠和全身性传播至脾脏，从而将沙门氏菌疫苗的功效扩展到家禽商品。对 BBS 866 疫苗接种的火鸡全血进行的转录分析显示，代谢和免疫基因（KCNAB1、ACOD1、GPR17、ADOR2AB 和 IL-17RD）下调，表明白细胞激活有限，接种疫苗后没有明显的外周炎症反应。
