Monson Melissa S, Gurung Manoj, Bearson Bradley L, Whelan Samuel J, Trachsel Julian M, Looft Torey, Sylte Matthew J, Bearson Shawn M D
Food Safety and Enteric Pathogens Research Unit, National Animal Disease Center, U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Ames, IA, United States.
Oak Ridge Institute for Science and Education, Agricultural Research Service (ARS) Research Participation Program, Oak Ridge, TN, United States.
Front Vet Sci. 2024 Dec 19;11:1502303. doi: 10.3389/fvets.2024.1502303. eCollection 2024.
Vaccines that cross-protect across serovars of () would be a beneficial intervention against emerging and persistent isolates of concern for the turkey industry. The 2017-2019 foodborne outbreak of serovar Reading (. Reading) revealed the need for effective control of this serovar in turkey production. This study evaluated two live-attenuated vaccines, an internally developed cross-protective vaccine and a commercially available vaccine, against an outbreak-associated strain of . Reading in turkeys. At 1 day and 3 weeks of age, male turkey poults were either mock-vaccinated with phosphate buffered saline (PBS) or given one of the vaccines by oral gavage (primary and booster) or aerosol spray (primary) then drinking water (booster). At 7 weeks of age, poults were challenged with 10 colony forming units (CFU) of . Reading; a mock-vaccinated group was mock-challenged with PBS. Colonization of the cecal contents and cecal tonsil was 1.5-3 log CFU/g lower in vaccinated birds than mock-vaccinated birds at 7 and/or 14 days post-inoculation (DPI). dissemination to the spleen was significantly reduced by both vaccines. Gene expression of intestinal transporters (such as and ) and tight junction proteins was significantly decreased in the turkey cecal tonsil transcriptome at 2 DPI with . Reading. Vaccination with either vaccine mitigated most cecal tonsil gene expression responses to . Reading challenge. Therefore, both the internally developed vaccine and commercial vaccine were cross-protective against colonization and dissemination, and both were able to limit transcriptional changes from challenge in intestinal health-related genes in the cecal tonsil, thereby providing vaccination efficacy and impact data against . Reading in turkeys.
能够对（）血清型提供交叉保护的疫苗将是一种有益的干预措施，可应对火鸡养殖业中出现的和持续存在的相关分离株。2017 - 2019年食源性雷丁血清型（. Reading）爆发事件表明，有必要在火鸡生产中有效控制该血清型。本研究评估了两种减毒活疫苗，一种是内部研发的具有交叉保护作用的疫苗和一种市售疫苗，用于抵抗火鸡中与疫情相关的雷丁血清型菌株。在1日龄和3周龄时，雄性小火鸡要么用磷酸盐缓冲盐水（PBS）进行 mock 疫苗接种，要么通过口服灌胃（初次和加强免疫）或喷雾（初次免疫）然后饮水（加强免疫）接种其中一种疫苗。在7周龄时，小火鸡用10个菌落形成单位（CFU）的雷丁血清型菌株进行攻毒；一个 mock 疫苗接种组用PBS进行 mock 攻毒。在接种后7天和/或14天，接种疫苗的鸡盲肠内容物和盲肠扁桃体的定殖量比 mock 疫苗接种的鸡低1.5 - 3 log CFU/g。两种疫苗均显著降低了雷丁血清型菌株向脾脏的传播。在接种雷丁血清型菌株后2天，火鸡盲肠扁桃体转录组中肠道转运蛋白（如和）和紧密连接蛋白的基因表达显著降低。接种任何一种疫苗都减轻了盲肠扁桃体对雷丁血清型菌株攻毒产生的大多数基因表达反应。因此，内部研发的疫苗和市售疫苗对定殖和传播均具有交叉保护作用，并且都能够限制盲肠扁桃体中与肠道健康相关基因因攻毒而产生的转录变化，从而提供了针对火鸡中雷丁血清型菌株的疫苗接种效果和影响数据。
