Energy Materials and Process, BK 21, Hanyang University, 17 Hangdang-dong, Seongdong-gu, Seoul 133-791, Republic of Korea.
Curr Microbiol. 2013 Jun;66(6):555-65. doi: 10.1007/s00284-013-0311-z. Epub 2013 Jan 29.
A(2)O process is a sequential wastewater treatment process that uses anaerobic, anoxic, and oxic chambers for nitrogen and phosphorus removal. In this study, the bacterial communities among these chambers were compared, and the diversity of the bacteria involved in nitrogen and phosphorus removal was surveyed. A pilot-scale A(2)O process (50 m(3) day(-1)) was operated for more than 6 months, and bacterial 16S rRNA gene diversity was analyzed using pyrosequencing. A total of 7,447 bacterial sequence reads were obtained from anaerobic (1,546), anoxic (2,158), and oxic (3,743) chambers. Even though there were differences in the atmospheric condition and functionality, no prominent differences could be found in the bacterial community of the three chambers of the pilot A(2)O process. All sequence reads, which were taxonomically analyzed using the Eztaxon-e database, were assigned into 638 approved or tentative genera. Among them, about 72.2 % of the taxa were contained in the phyla Proteobacteria and Bacteroidetes. Phosphate-accumulating bacteria, Candidatus Accumulibacter phosphatis, and two other Accumulibacter were found to constitute 3.1 % of the identified genera. Ammonia-oxidizing bacteria, Nitrosomonas oligotropha, and four other phylotypes in the same family, Nitrosomonadaceae, constituted 0.2 and 0.9 %, respectively. Nitrite-oxidizing bacteria, Nitrospira defluvii, and other three phylotypes in the same family, Nitrospiraceae, constituted 2.5 and 0.1 %, respectively. In addition, Dokdonella and a phylotype of the phylum Chloroflexi, function in nitrogen and/or phosphate removal of which have not been reported in the A(2)O process, constituted the first and third composition among genera at 4.3 and 3.8 %, respectively.
A(2)O 工艺是一种顺序式污水处理工艺，利用厌氧、缺氧和有氧室进行氮磷去除。本研究比较了这些室中的细菌群落，并调查了参与氮磷去除的细菌多样性。一个中试规模的 A(2)O 工艺（50 m(3)天(-1)) 运行了 6 个多月，使用焦磷酸测序法分析了细菌 16S rRNA 基因多样性。从厌氧（1,546）、缺氧（2,158）和好氧（3,743）室获得了总共 7,447 个细菌序列读段。尽管三个室的大气条件和功能不同，但在中试 A(2)O 工艺的三个室的细菌群落中没有发现明显的差异。使用 Eztaxon-e 数据库进行分类分析的所有序列读段都被分配到 638 个已批准或暂定的属中。其中，约 72.2%的类群包含在 Proteobacteria 和 Bacteroidetes 门中。聚磷菌、Candidatus Accumulibacter phosphatis 和另外两种 Accumulibacter 被发现构成了所鉴定属的 3.1%。氨氧化菌、Nitrosomonas oligotropha 和同一科的另外四个菌型（Nitrosomonadaceae）构成了 0.2%和 0.9%。亚硝酸盐氧化菌、Nitrospira defluvii 和同一科的另外三个菌型（Nitrospiraceae）构成了 2.5%和 0.1%。此外，Dokdonella 和一个 Chloroflexi 门的菌型，其在 A(2)O 工艺中去除氮和/或磷的功能尚未报道，分别构成了属中的第一和第三组成部分，占 4.3%和 3.8%。
