Zamanian-Azodi Mona, Rezaei-Tavirani Mostafa, Mahboubi Mohammad, Hamidpour Mohsen, Rezaei Tavirani Majid, Hamdieh Mostafa, Rostami-Nejad Mohammad, Nejadi Naser, Derakhshan Mohammad Kamran
National Elites Foundation, Tehran, Iran.
Proteomics Research Center, School of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Basic Clin Neurosci. 2018 Sep-Oct;9(5):337-346. doi: 10.32598/bcn.9.5.337. Epub 2018 Sep 1.
Many genetic studies are conducted on Obsessive-Compulsive Disorder (OCD). however, a high-throughput examination of proteome profile of this severe disease has not been performed yet.
Here, the proteomic study of OCD patients' serum samples was conducted by the application of Two-Dimensional Electrophoresis (2DE) followed by Mass Spectrometry (MALDI-TOF-TOF).
A total of 240 protein spots were detected and among them, five significant differentially expressed protein spots with the fold change of ≥1.5 were considered for further evaluations. These proteins include IGKC, GC, HPX, and two isoforms of HP. While IGKC and HP show down-regulation, GC and HPX indicate up-regulation. Moreover, a validation study of overall HP levels in patients' serum via nephelometric quantification confirmed the lower levels of this protein in the serum of OCD patients. Additionally, enrichment analysis and validation test revealed that inflammation is one of most dominant processes in OCD.
It is suggested that these candidate proteins and their underlying processes (especially, inflammation) may be linked to OCD pathophysiology and can promise a clinical use after extensive validation studies.
许多基因研究都围绕强迫症（OCD）展开。然而，尚未对这种严重疾病的蛋白质组图谱进行高通量检测。
在此，通过二维电泳（2DE）结合基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-TOF）对强迫症患者的血清样本进行蛋白质组学研究。
共检测到240个蛋白质斑点，其中有5个显著差异表达的蛋白质斑点，其倍数变化≥1.5，被纳入进一步评估。这些蛋白质包括免疫球蛋白κ链（IGKC）、维生素D结合蛋白（GC）、触珠蛋白（HPX）以及触珠蛋白（HP）的两种亚型。IGKC和HP呈下调趋势，而GC和HPX呈上调趋势。此外，通过比浊定量法对患者血清中HP的总体水平进行验证研究，证实强迫症患者血清中该蛋白水平较低。另外，富集分析和验证试验表明，炎症是强迫症中最主要的过程之一。
这些候选蛋白质及其潜在过程（尤其是炎症）可能与强迫症的病理生理学相关，有望在经过广泛验证研究后应用于临床。
