Zamanian Azodi Mona, Rezaei Tavirani Mostafa, Arefi Oskouie Afsaneh, Hamdieh Mostafa, Derakhshan Mohammad Kamran, Ahmadzadeh Alireza, Zayeri Farid, Nejadi Naser, Rezaei Tavirani Majid, Mansouri Vahid, Rostami-Nejad Mohammad, Vafaee Reza
Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Psychosomatic Department, Taleghani Hospital, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Iran J Pharm Res. 2017 Summer;16(3):1264-1271.
Obsessive-Compulsive Disorder (OCD) is one of the most common mental conditions. Proteome profiling may help identifying important proteins and finally shed lights to complexity of OCD underlying mechanisms. Here, by the application gel-based proteomic approach the proteome profile of patients with washing subtype of OCD before and after treatment with Fluoxetine (positive responders) are compared to healthy matched controls. However, only one of the differentially expressed proteins is examined and introduced in this paper. Proteomic analysis was done by the application of two-dimensional polyacrylamide gel electrophoresis (2-D PAGE), combined with (MALDI-TOF-TOF MS)-based. Furthermore, network analysis and biological annotation were handled by Cytoscape Plug-in and CluePedia. The proteome comparison between groups identified protein with the significant expression changes (p<0.05 and fold change ≥ 1.5). While the expression level of Ig Kappa Chain C Region is significantly decreased in OCD patients before any treatments, the trend is almost normalized after treatment with Fluoxetine in positive responders. In addition, interaction profile of IGKC shows that the interacting proteins may be affected as the expression pattern of IGKC changes in OCD patients. In conclusion, IGKC may be introduced as potential biomarker in our study; yet, investigation in bigger sample size and application of validation methods is a requirement.
强迫症（OCD）是最常见的精神疾病之一。蛋白质组分析可能有助于识别重要蛋白质，并最终揭示强迫症潜在机制的复杂性。在此，通过基于凝胶的蛋白质组学方法，将强迫症洗涤亚型患者在使用氟西汀治疗前后（阳性反应者）的蛋白质组图谱与健康匹配对照进行比较。然而，本文仅研究并介绍了一种差异表达蛋白。蛋白质组分析通过二维聚丙烯酰胺凝胶电泳（2-D PAGE）结合基于基质辅助激光解吸电离飞行时间串联质谱（MALDI-TOF-TOF MS）进行。此外，网络分析和生物学注释由Cytoscape插件和CluePedia处理。组间蛋白质组比较确定了具有显著表达变化的蛋白质（p<0.05且变化倍数≥1.5）。在任何治疗前，强迫症患者中Igκ链C区的表达水平显著降低，而在阳性反应者中，使用氟西汀治疗后该趋势几乎恢复正常。此外，IGKC的相互作用图谱表明，随着强迫症患者中IGKC表达模式的变化，相互作用蛋白可能会受到影响。总之，在我们的研究中，IGKC可能被作为潜在的生物标志物；然而，需要更大样本量的研究和验证方法的应用。
