Etesam Zahra, Nemati Maryam, Ebrahimizadeh Mohammad-Amin, Ebrahimi Hossain-Ali, Hajghani Hossain, Khalili Tahereh, Jafarzadeh Abdollah
Neurology Research Center, Kerman University of Medical Sciences, Kerman, Iran.
Department of Immunology, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
Basic Clin Neurosci. 2018 Nov-Dec;9(6):458-469. doi: 10.32598/bcn.9.6.458. Epub 2018 Nov 1.
Multiple Sclerosis (MS) is an inflammatory disorder caused by self-reactive Th1 lymphocytes, while Th2 cells may confer protection. The Th1 and Th2 cell differentiation are regulated by specific transcription factors, especially and , respectively. This investigation aimed to measure the and expression by Peripheral Blood Mononuclear Cells (PBMCs) obtained from MS patients after specific and non-specific in vitro stimulation.
The PBMCs were separated from 22 patients with MS and 20 healthy individuals. They were cultured at 37°C for 24 h in the absence of a stimulator or in the presence of Myelin oligodendrocyte Glycoprotein (MOG) or Phytohemagglutinin (PHA) at a concentration of 10 μg/mL. Then the and expression was measured by real time-PCR.
The expression was enhanced, while the expression diminished. Therefore the expression of / ratio diminished in not-stimulated, MOG-stimulated and PHA-stimulated PBMCs from MS patients compared with equal cultures from the healthy individuals (P<0.01, P<0.01 and P<0.01, for ; P<0.03, P<0.01 and P<0.02, for ; P<0.01, P<0.001 and P<0.01 for / ratio, respectively). The not-stimulated, MOG-stimulated, and PHA-stimulated PBMCs from men with MS expressed higher amounts of than equal cells from MS women (P<0.05, P<0.05 and P<0.01, respectively).
These results probably indicate an imbalance in Th1/Th2 cells in the level of transcription factors with a tendency toward Th1 cells in MS. The clinical utilization of the transcription factors as novel biomarkers of MS should be evaluated in further studies.
多发性硬化症（MS）是一种由自身反应性Th1淋巴细胞引起的炎症性疾病，而Th2细胞可能具有保护作用。Th1和Th2细胞的分化分别受特定转录因子的调控，尤其是 和 。本研究旨在检测多发性硬化症患者外周血单个核细胞（PBMC）在特异性和非特异性体外刺激后 和 的表达情况。
从22例多发性硬化症患者和20名健康个体中分离出PBMC。将其在37°C下培养24小时，培养时分别不添加刺激物、添加浓度为10μg/mL的髓鞘少突胶质细胞糖蛋白（MOG）或植物血凝素（PHA）。然后通过实时定量PCR检测 和 的表达。
的表达增强，而 的表达减弱。因此，与健康个体的同等培养物相比，多发性硬化症患者未刺激、MOG刺激和PHA刺激的PBMC中 / 比值的表达降低（ 分别为P<0.01、P<0.01和P<0.01； 分别为P<0.03、P<0.01和P<0.02； / 比值分别为P<0.01、P<0.001和P<0.01）。多发性硬化症男性患者未刺激、MOG刺激和PHA刺激的PBMC中 的表达量高于多发性硬化症女性患者的同等细胞（分别为P<0.05、P<0.05和P<0.01）。
这些结果可能表明在转录因子水平上Th1/Th2细胞失衡，多发性硬化症患者倾向于Th1细胞。转录因子作为多发性硬化症新生物标志物的临床应用有待进一步研究评估。
