Reitz Annalena, Poppert Sven, Rieker Melanie, Frickmann Hagen
University Hospital of the Goethe University, Frankfurt/Main, Germany.
Swiss Tropical and Public Health Institute, Basel, Switzerland.
Eur J Microbiol Immunol (Bp). 2018 Dec 12;8(4):135-141. doi: 10.1556/1886.2018.00024. eCollection 2018 Dec 23.
The study assessed a spectrum of previously published in-house fluorescence in-situ hybridization (FISH) probes in a combined approach regarding their diagnostic performance with incubated blood culture materials.
Within a two-year interval, positive blood culture materials were assessed with Gram and FISH staining. Previously described and new FISH probes were combined to panels for Gram-positive cocci in grape-like clusters and in chains, as well as for Gram-negative rod-shaped bacteria. Covered pathogens comprised spp., such as spp., spp., including , and spp., like , and , such as and spp., , and spp.
A total of 955 blood culture materials were assessed with FISH. In 21 (2.2%) instances, FISH reaction led to non-interpretable results. With few exemptions, the tested FISH probes showed acceptable test characteristics even in the routine setting, with a sensitivity ranging from 28.6% spp.) to 100% (6 probes) and a specificity of >95% in all instances.
If sophisticated rapid diagnostic methods like mass spectrometry from blood culture materials are not available, FISH provides an option for rapid differentiation for laboratories in resource-limited settings.
本研究采用联合方法评估了一系列先前发表的内部荧光原位杂交（FISH）探针与培养的血培养材料的诊断性能。
在两年的时间间隔内，对阳性血培养材料进行革兰氏染色和FISH染色评估。将先前描述的和新的FISH探针组合成用于葡萄状簇状和链状革兰氏阳性球菌以及革兰氏阴性杆状细菌的检测板。涵盖的病原体包括 属，如 属、 属，包括 ，以及 属，如 、 ，以及 属，如 和 属、 ，以及 属。
共对955份血培养材料进行了FISH评估。在21例（2.2%）中，FISH反应导致结果无法解读。除少数例外情况外，即使在常规环境中，所测试的FISH探针也显示出可接受的检测特性，灵敏度范围为28.6%（ 属）至100%（6种探针），所有情况下特异性均>95%。
如果无法获得像从血培养材料中进行质谱分析这样复杂的快速诊断方法，FISH为资源有限环境中的实验室提供了一种快速鉴别选择。
