Sahin Kazim, Orhan Cemal, Tuzcu Mehmet, Tastan Hakki, Bilir Birdal, Sahin Nurhan, Oner Deniz Aslar, Kucuk Omer
Department of Animal Nutrition, Faculty of Veterinary Medicine, Firat University, Elazig, Turkey.
Department of Biology, Faculty of Science, Firat University, Elazig, Turkey.
J Aging Res. 2019 Jan 2;2019:1643243. doi: 10.1155/2019/1643243. eCollection 2019.
In the present study, we aimed to investigate the effects of tomato powder (TP) on glucose and lipid metabolism, as well as oxidative stress and the NF-B, mTOR, and Nrf2 pathways during the aging process in healthy rats.
Male Wistar rats were randomly assigned to four groups as follows: (i) Control group 1 (=15, 3-week old): rats were fed standard diet for 7 weeks; (ii) TP group 1 (=15, 3-week old): rats were fed standard diet supplemented with TP for 7 weeks; (iii) Control group 2 (=15, 8-week old): rats were fed standard diet for 69 weeks; and (iv) TP group 2 (8-week old): rats were fed standard diet supplemented with TP for 69 weeks. TP supplementation significantly reduced the hyperglycemia, hypertriglyceridemia, and hypercholesterolemia and improved liver function and kidney function in 77-week old rats compared with the control animals ( < 0.05). In addition, TP significantly decreased the serum and liver MDA levels ( < 0.003 and < 0.001, respectively) while increasing the activities of liver SOD ( < 0.001), CAT ( < 0.008), and GPx ( < 0.01) compared with the control groups in both 10-week-old and 77-week-old rats ( < 0.05). Age-related increases in phosphorylation of NF-Bp65, mTOR, 4E-BP1, and P70S6K were observed in livers of 77-week-old rats compared to those of 10-week-old rats ( < 0.001). TP supplementation decreased the expression of NF-Bp65 and activation of mTOR, 4E-BP1, and P70S6K in livers of 77-week-old rats compared to the control animals. Moreover, TP supplementation significantly elevated Nrf2 expression in livers of both 10-week-old and 77-week-old rats ( < 0.05).
TP ameliorates age-associated inflammation and oxidative stress through the inhibition of NF-Bp65, mTOR pathways, and Nrf2 activation may explain the observed improvement in glucose and lipid metabolism as well as the improved liver and kidney functions.
在本研究中，我们旨在探究番茄粉（TP）对健康大鼠衰老过程中葡萄糖和脂质代谢、氧化应激以及NF-κB、mTOR和Nrf2信号通路的影响。
雄性Wistar大鼠随机分为四组如下：（i）对照组1（n = 15，3周龄）：大鼠喂养标准饮食7周；（ii）TP组1（n = 15，3周龄）：大鼠喂养补充TP的标准饮食7周；（iii）对照组2（n = 15，8周龄）：大鼠喂养标准饮食69周；（iv）TP组2（n = 15，8周龄）：大鼠喂养补充TP的标准饮食69周。与对照动物相比，补充TP显著降低了77周龄大鼠的高血糖、高甘油三酯血症和高胆固醇血症，并改善了肝功能和肾功能（P < 0.05）。此外，与10周龄和77周龄大鼠的对照组相比，TP显著降低了血清和肝脏MDA水平（分别为P < 0.003和P < 0.001），同时增加了肝脏SOD（P < 0.001）、CAT（P < 0.008）和GPx（P < 0.01）的活性（P < 0.05）。与10周龄大鼠相比，77周龄大鼠肝脏中观察到与年龄相关的NF-κBp65、mTOR、4E-BP1和P70S6K磷酸化增加（P < 0.001）。与对照动物相比，补充TP降低了77周龄大鼠肝脏中NF-κBp65的表达以及mTOR、4E-BP1和P70S6K的激活。此外，补充TP显著提高了10周龄和77周龄大鼠肝脏中Nrf2的表达（P < 0.05）。
TP通过抑制NF-κBp65、mTOR信号通路改善与年龄相关的炎症和氧化应激，Nrf2激活可能解释了观察到的葡萄糖和脂质代谢改善以及肝脏和肾脏功能的改善。
