Department of Periodontology, Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatology Hospital of Guangzhou Medical University, Guangzhou, China.
Department of Prosthodontics, Guanghua School of Stomatology, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, China.
Biomed Res Int. 2019 Jan 3;2019:9042542. doi: 10.1155/2019/9042542. eCollection 2019.
This study aimed to explore periodontal and systemic immune response of overweight hosts to periodontitis. Forty C57 BL/6J male mice were divided into high (HF) or low fat (LF) diet groups and fed with the two diets, respectively, for 8 weeks. Each diet group was then divided into periodontitis (P) or control (C) groups (n = 10 per group) for 10-day ligation or sham-ligation. Overweight-related parameters including body weight were measured. Alveolar bone loss (ABL) was morphometrically analyzed and periodontal osteoclasts were stained. Periodontal immune response including leukocyte and macrophage number and inflammatory cytokines were analyzed by histology and quantitative PCR. Serum cytokine and lipid levels were quantified using electrochemiluminescence immunoassays, enzyme-linked immunosorbent assays, and biochemistry. It was found that HF group had 14.4% body weight gain compared with LF group ( < 0.01). ABL and periodontal osteoclast, leukocyte, and macrophage number were higher in P group than C group regardless of diet ( < 0.05). ABL and periodontal osteoclast number were not affected by diet regardless of ligation or sham-ligation. Leukocyte and macrophage number and protein level of tumor necrosis factor (TNF-) in periodontium and serum interleukin-6 level were downregulated by HF diet in periodontitis mice ( < 0.05). Periodontal protein level of TNF- was highly correlated with serum interleukin-6 and low-density lipoprotein cholesterol levels ( < 0.01). These findings indicated that impaired immune response occurs both periodontally and systemically in preobesity overweight individuals. Given a well-reported exacerbating effect of obesity on periodontitis, overweight, if let uncontrolled, might place the individuals at potential risk for future periodontal tissue damage.
本研究旨在探讨超重宿主牙周炎的牙周和全身免疫反应。将 40 只 C57BL/6J 雄性小鼠分为高脂肪（HF）或低脂肪（LF）饮食组，并分别用两种饮食喂养 8 周。然后，每个饮食组再分为牙周炎（P）或对照（C）组（每组 n = 10）进行 10 天结扎或假结扎。测量与超重相关的参数，包括体重。通过形态计量学分析牙槽骨丧失（ABL）并染色牙周破骨细胞。通过组织学和定量 PCR 分析牙周免疫反应，包括白细胞和巨噬细胞数量和炎症细胞因子。使用电化学发光免疫分析、酶联免疫吸附测定和生化方法定量测定血清细胞因子和脂质水平。结果发现，HF 组体重比 LF 组增加了 14.4%（<0.01）。无论饮食如何，P 组的 ABL 和牙周破骨细胞、白细胞和巨噬细胞数量均高于 C 组（<0.05）。无论结扎与否，ABL 和牙周破骨细胞数量不受饮食影响。在牙周炎小鼠中，HF 饮食下调了牙周组织和血清白细胞介素-6 水平的 TNF- 蛋白水平（<0.05）。在牙周炎小鼠中，HF 饮食下调了牙周组织和血清白细胞介素-6 水平的 TNF- 蛋白水平（<0.05）。牙周组织 TNF- 蛋白水平与血清白细胞介素-6 和低密度脂蛋白胆固醇水平高度相关（<0.01）。这些发现表明，在肥胖前期超重个体中，牙周和全身的免疫反应均受到损害。鉴于肥胖对牙周炎的恶化作用已有充分报道，如果不加以控制，超重可能会使个体面临未来牙周组织损伤的潜在风险。
