Department of Neck Surgery, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China.
Department of Immunology, Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China.
Oncol Rep. 2019 Apr;41(4):2511-2517. doi: 10.3892/or.2019.6996. Epub 2019 Feb 1.
As a common malignancy of the endocrine system, papillary thyroid carcinoma (PTC) seriously affects the quality of life of patients. lncRNA PAX8‑AS1:28, or lnc‑PSD4‑1:14 has been reported to be abnormally expressed in PTC. However, the function of PAX8‑AS1:28 in PTC is still unknown. Therefore, the present study aimed to investigate the functions of PAX8‑AS1:28 in PTC, and to explore the possible mechanisms of action. A total of 38 patients with PTC were included and the normal thyroid follicular epithelial cell line Nthy‑ori 3‑1 and PTC cell line IHH‑4 were also used. MYC and PAX8‑AS1:28 overexpression and siRNA silencing in the cell lines were carried out. Expression of PAX8‑AS1:28, PAX8 and MYC in tumor tissue, adjacent healthy tissue and different cell lines were detected by qRT‑PCR and western blot analysis. Cell proliferation was measured by CCK‑8 assay. Expression levels of PAX8‑AS1:28 and PAX8 were lower in PTC tumor tissue and PTC cells than those in healthy tissue and normal cells. In contrast, the expression level of MYC was higher in PTC cells than that in normal cells. PAX8‑AS1:28 silencing reduced the expression level of PAX8 and promoted tumor cell growth, while PAX8‑AS1:28 overexpression increased the expression level of PAX8 and inhibited tumor cell growth. MYC silencing increased expression levels of PAX8‑AS1:28 and PAX8 and inhibited tumor cell growth, while MYC overexpression decreased expression levels of PAX8‑AS1:28 and PAX8 and promoted tumor cell growth. MYC can promote PTC by inhibiting the expression of lncRNA PAX8‑AS1:28.
作为内分泌系统的常见恶性肿瘤，甲状腺乳头状癌（PTC）严重影响患者的生活质量。lncRNA PAX8-AS1:28 或 lnc-PSD4-1:14 已被报道在 PTC 中异常表达。然而，PAX8-AS1:28 在 PTC 中的功能仍不清楚。因此，本研究旨在探讨 PAX8-AS1:28 在 PTC 中的功能，并探讨其可能的作用机制。共纳入 38 例 PTC 患者，同时使用正常甲状腺滤泡上皮细胞系 Nthy-ori 3-1 和 PTC 细胞系 IHH-4。在细胞系中进行 MYC 和 PAX8-AS1:28 的过表达和 siRNA 沉默。通过 qRT-PCR 和 Western blot 分析检测肿瘤组织、相邻正常组织和不同细胞系中 PAX8-AS1:28、PAX8 和 MYC 的表达。通过 CCK-8 测定法测量细胞增殖。与正常细胞相比，PTC 肿瘤组织和 PTC 细胞中 PAX8-AS1:28 和 PAX8 的表达水平较低，而 MYC 的表达水平较高。沉默 PAX8-AS1:28 降低了 PAX8 的表达水平并促进了肿瘤细胞的生长，而过表达 PAX8-AS1:28 则增加了 PAX8 的表达水平并抑制了肿瘤细胞的生长。沉默 MYC 增加了 PAX8-AS1:28 和 PAX8 的表达水平并抑制了肿瘤细胞的生长，而过表达 MYC 降低了 PAX8-AS1:28 和 PAX8 的表达水平并促进了肿瘤细胞的生长。MYC 可以通过抑制 lncRNA PAX8-AS1:28 的表达来促进 PTC。
