Hu Jialei, Mu Hanyou, Gao Linshan, Pan Ying, Wu Chuanfei, Zhang Dong, Chen Qiaojun, Ding Honghui
Clinical Laboratory, Yiwu Maternal and Child Health Hospital, Jinhua, Zhejiang 321000, P.R. China.
Department of Pediatrics, Yiwu Maternal and Child Health Hospital, Jinhua, Zhejiang 321000, P.R. China.
Exp Ther Med. 2021 Feb;21(2):145. doi: 10.3892/etm.2020.9576. Epub 2020 Dec 16.
Noncoding RNAs (ncRNAs) are involved in the pathological processes of various diseases. The aim of the present study was to verify the expression levels and the diagnostic value of two candidate ncRNAs in the blood leukocytes of patients with gestational diabetes mellitus (GDM) compared to healthy controls. The long ncRNA paired box 8 antisense 1 (Pax8-AS1) and the microRNA miR-4646 were selected, which were identified to be associated with GDM by bioinformatics analysis of a dataset from the Gene Expression Omnibus GEO database. By using reverse transcription-quantitative PCR, the expression levels of Pax8-AS1 and miR-4646 were analysed in leukocytes of patients with GDM (n=35) and normal pregnant females (n=35). The results indicated a significant decrease in the expression levels of both Pax8-AS1 and miR-4646 in patients with GDM as compared with those in the healthy controls. In the second trimester, a strong negative correlation between Pax8-AS1/miR-4646 and 2-h glucose levels was detected in patients with GDM. Receiver operating characteristic curve analysis indicated that the levels of Pax8-AS1 and miR-4646 in the second trimester of pregnancy had a significant diagnostic value with high selectivity and specificity for GDM (area under the curve values, 0.902 and 0.891, respectively; P<0.001). Overall, the present study suggested that Pax8-AS1 and miR-4646 may serve as promising diagnostic biomarkers for GDM.
非编码RNA（ncRNAs）参与多种疾病的病理过程。本研究的目的是验证与健康对照相比，两种候选ncRNAs在妊娠期糖尿病（GDM）患者血液白细胞中的表达水平及诊断价值。选择了长链ncRNA配对盒8反义1（Pax8-AS1）和微小RNA miR-4646，通过对来自基因表达综合数据库GEO数据库的数据集进行生物信息学分析，确定它们与GDM相关。采用逆转录定量PCR法，分析了35例GDM患者和35例正常妊娠女性白细胞中Pax8-AS1和miR-4646的表达水平。结果表明，与健康对照相比，GDM患者中Pax8-AS1和miR-4646的表达水平均显著降低。在孕中期，GDM患者中检测到Pax8-AS1/miR-4646与2小时血糖水平呈强负相关。受试者工作特征曲线分析表明，妊娠中期Pax8-AS1和miR-4646水平对GDM具有显著的诊断价值，具有较高的选择性和特异性（曲线下面积值分别为0.902和0.891；P<0.001）。总体而言，本研究表明Pax8-AS1和miR-4646可能是GDM有前景的诊断生物标志物。
