微小 RNA-425-5p 通过靶向叉头框 J3 促进前列腺癌的发展。

MicroRNA-425-5p promotes the development of prostate cancer via targeting forkhead box J3.

机构信息

Department of Urology, the Second Hospital of Fujian Medical University, 34 North Zhongshan Road, Quanzhou, P. R. China.

出版信息

Eur Rev Med Pharmacol Sci. 2019 Jan;23(2):547-554. doi: 10.26355/eurrev_201901_16867.

DOI:10.26355/eurrev_201901_16867

PMID:30720162

Abstract

OBJECTIVE

MicroRNAs (miRNAs) have critical roles in the progression of prostate cancer (PCa) and have the potential to be used as prognosis biomarkers. In this study, we aimed to investigate the role of miR-425-5p in the progression of PCa.

PATIENTS AND METHODS

miR-425-5p expression in PCa tumor tissues and cell lines was measured by Quantitative Real-time PCR (RT-qPCR). Effects of miR-425-5p expression on PCa cell proliferation, colony formation, cell migration, and cell invasion were measured.

RESULTS

We found miR-425-5p expression was elevated in both PCa tissues and cell lines. Importantly, we found overexpression of miR-425-5p promoted proliferation, colony formation, migration and invasion of PCa cell lines in vitro. Forkhead box J3 (FOXJ3) was validated as a downstream target of miR-425-5p. Finally, we found the stimulation effects of miR-425-5p on PCa cell behaviors were fulfilled through directly regulating the expression of FOXJ3, which validated FOXJ3 as a functional target of miR-425-5p in PCa.

CONCLUSIONS

Taken together, our results demonstrated miR-425-5p may contribute the malignancy progression of PCa in a mechanism involving FOXJ3, implicating miR-425-5p may be developed as therapeutic target for PCa in the future.

摘要

目的

MicroRNAs（miRNAs）在前列腺癌（PCa）的进展中起着关键作用，具有作为预后生物标志物的潜力。在这项研究中，我们旨在研究 miR-425-5p 在 PCa 进展中的作用。

患者和方法

通过定量实时 PCR（RT-qPCR）测量 PCa 肿瘤组织和细胞系中的 miR-425-5p 表达。测量 miR-425-5p 表达对 PCa 细胞增殖、集落形成、细胞迁移和细胞侵袭的影响。

结果

我们发现 miR-425-5p 在 PCa 组织和细胞系中均上调。重要的是，我们发现 miR-425-5p 的过表达促进了体外 PCa 细胞系的增殖、集落形成、迁移和侵袭。叉头框 J3（FOXJ3）被验证为 miR-425-5p 的下游靶标。最后，我们发现 miR-425-5p 对 PCa 细胞行为的刺激作用是通过直接调节 FOXJ3 的表达来实现的，这验证了 FOXJ3 是 miR-425-5p 在 PCa 中的功能靶标。