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[质谱法检测霍乱弧菌毒力标志物。]

[Mass spectrometry virulence marker Vibrio cholerae.].

作者信息

Chaika S O, Telesmanich N R, Lomov Yu M

机构信息

Rostov-on-Don State Medical University, chair of biochemistry №1, 344022, Rostov-on-Don, Russian Federation.

出版信息

Klin Lab Diagn. 2018;63(7):445-449. doi: 10.18821/0869-2084-2018-63-7-445-449.

Abstract

Identification mass-spectrometry of marker proteins of toxicity of V. cholerae for development the express of diagnostics of the causative agent of cholera on the basis of the computer analysis in the MALDI-ToF format of electronic profiles became the purpose of our research. Subjected to the computer analysis mass and spectrometer electronic passports 140 of strains of V. cholerae with obviously known characteristic of strains which were divided into 2 basic groups in the parameter of existence or lack of a gene of cholera toxin (ctx-and ctx+). We for the first time revealed a taxon - the specific marker protein ceous peak having a molecular mass of 3202 Da characteristic of strains of V.cholerae ctx + and unrepresentative for V.cholerae ctx-.

摘要

基于计算机分析的基质辅助激光解吸电离飞行时间(MALDI-ToF)格式电子图谱,对霍乱弧菌毒性标记蛋白进行鉴定质谱分析,以开发霍乱病原体的快速诊断方法,这成为我们研究的目的。对140株霍乱弧菌菌株的质谱和光谱电子图谱进行了计算机分析,这些菌株具有明显已知的菌株特征,根据霍乱毒素基因(ctx+和ctx-)的存在与否参数分为2个基本组。我们首次发现了一个分类群——一种特定的标记蛋白峰,其分子量为3202 Da,是ctx+霍乱弧菌菌株的特征,而ctx-霍乱弧菌菌株不具有。

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