Denari J H, Rosner J M
Int J Fertil. 1978;23(2):123-7.
Previous work in our laboratory has demonstrated the specific synthesis of a soluble protein (A) in the rat endometrium on day L4 of pseudopregnancy 1 h after the decidual induction; the A protein synthesis being higher in the antimesometrial region (AMR) than in the mesometrial one. We have now examined in the AMR of rat endometria on day L4 of pseudopregnancy: 1) the effect of cycloheximide (500 microgram, i.p. 30 min before decidual induction) on the biosynthesis of the A protein, and 2) the effect of actinomycin D (Act. D) (5 microgram/uterine horn 60 min before the decidual stimulation) on the induction of A protein synthesis. The synthesis of the A protein was determined by acrylamide gel electrophoresis of endometrial soluble proteins after double-labeling incorporation of amino acids. Results showed that: 1) the cycloheximide inhibited the synthesis of the A protein which indicates a de novo protein synthesis; 2) the Act. D inhibited the A synthesis suggesting that synthesis of an Act. D-sensitive product is one of the earliest macromolecular synthetic events after decidual induction.
我们实验室之前的研究表明，在假孕第4天蜕膜诱导后1小时，大鼠子宫内膜会特异性合成一种可溶性蛋白（A）；抗中膜区域（AMR）中A蛋白的合成高于中膜区域。我们现在研究了假孕第4天大鼠子宫内膜AMR中：1）放线菌酮（500微克，在蜕膜诱导前30分钟腹腔注射）对A蛋白生物合成的影响，以及2）放线菌素D（Act. D）（5微克/子宫角，在蜕膜刺激前60分钟）对A蛋白合成诱导的影响。通过对氨基酸进行双标记掺入后对子宫内膜可溶性蛋白进行丙烯酰胺凝胶电泳来测定A蛋白的合成。结果显示：1）放线菌酮抑制了A蛋白的合成，这表明是从头合成蛋白质；2）Act. D抑制了A蛋白的合成，提示合成一种对Act. D敏感的产物是蜕膜诱导后最早的大分子合成事件之一。
