Guan Jian, Li Yao, Ding Luo-Bin, Liu Guang-Yuan, Zheng Xiao-Fei, Xue Wei, Wang Hua-Jun
Department of Orthopedic Surgery, Third Hospital of Shijiazhuang, Shijiazhuang, Hebei 050000, China.
Department of Orthopedic Surgery and Sports Medicine Center, First Affiliated Hospital of Jinan University, Guangzhou, Guangdong 510632, China.
Discov Med. 2019 Jan;27(146):17-26.
The current study was carried out to investigate the serum and synovial fluid (SF) alpha-melanocyte-stimulating hormone (α-MSH) levels in correlation with disease severity in primary knee osteoarthritis (OA).
This study comprised of 105 primary knee OA patients and 98 healthy controls. The radiographic severity was verified according to the Kellgren-Lawrence radiographic grading criteria. The symptomatic severity of knee OA was assessed by the Western Ontario and McMaster Universities (WOMAC) Osteoarthritis Index. Serum α-MSH concentrations were measured by ELISA. The inflammation markers IL-6 and TNF-α, as well as cartilage damage markers MMP-3 (matrix metalloproteinase 3) and COMP (cartilage oligomeric matrix protein), were also measured. The receiver operating characteristic (ROC) curve was used to evaluate the diagnostic value between α-MSH and other four markers with regard to the radiographic progression.
SF α-MSH concentrations were negatively related to Kellgren-Lawrence grades and WOMAC index. SF α-MSH levels in knee OA patients were negatively associated with inflammation markers IL-6, TNF-α, and cartilage damage factors COMP and MMP-3. In addition, ROC analysis implied that attenuated α-MSH levels may serve as a favorable diagnostic marker for the radiographic progression. The difference of serum α-MSH concentration was not significant between knee OA patients and healthy controls.
Reduced SF α-MSH expression may be a characteristic of OA patients. Attenuated α-MSH level in SF may serve as a potential biomarker for disease severity of knee OA, and further studies are needed to identify its potential application for monitoring the course of the disease and the efficacy of therapies in OA patients.
开展本研究以调查原发性膝关节骨关节炎（OA）患者血清和滑液（SF）中α-黑素细胞刺激素（α-MSH）水平与疾病严重程度的相关性。
本研究纳入了105例原发性膝关节OA患者和98例健康对照者。根据Kellgren-Lawrence放射学分级标准确定放射学严重程度。采用西安大略和麦克马斯特大学（WOMAC）骨关节炎指数评估膝关节OA的症状严重程度。通过酶联免疫吸附测定法（ELISA）检测血清α-MSH浓度。还检测了炎症标志物白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α），以及软骨损伤标志物基质金属蛋白酶3（MMP-3）和软骨寡聚基质蛋白（COMP）。采用受试者工作特征（ROC）曲线评估α-MSH与其他四种标志物在放射学进展方面的诊断价值。
SF中α-MSH浓度与Kellgren-Lawrence分级和WOMAC指数呈负相关。膝关节OA患者的SF中α-MSH水平与炎症标志物IL-6、TNF-α以及软骨损伤因子COMP和MMP-3呈负相关。此外，ROC分析表明，α-MSH水平降低可能是放射学进展的良好诊断标志物。膝关节OA患者与健康对照者血清α-MSH浓度差异无统计学意义。
SF中α-MSH表达降低可能是OA患者的一个特征。SF中α-MSH水平降低可能是膝关节OA疾病严重程度的潜在生物标志物，需要进一步研究以确定其在监测OA患者疾病进程和治疗疗效方面的潜在应用。
