Bai Zhangzhen, Yu Rui, Zheng Tiantian, Sun Daoyang, Zhou Yang, Tang Junman, Zhu Huili, Li Guangning, Niu Lixin, Cui Lu, Du Rui, Zhang Jing, Zhang Yanlong
National Engineering Technology Research Center for Oil Peony, College of Landscape Architecture and Arts, Northwest A&F University, Yangling 712100, China.
College of Horticulture, China Agricultural University, Beijing 100193, China.
Metabolites. 2022 Apr 4;12(4):326. doi: 10.3390/metabo12040326.
Gallotannins (GTs) are a series of hydrolyzable tannins with multiple health-promoting effects. In this study, an integrated liquid chromatography tandem mass spectrometry (LC-MS/MS) strategy was developed for unveiling the spatial distribution pattern of GTs in the emerging oilseed crops and . According to the fragmentation behavior of the representative GT (1,2,3,4,6-penta--galloyl-β--glucose, PGG), the diagnostic neutral loss (NL) of 170 Da was chosen for the non-targeted screening of GT precursors. Simultaneously, the tandem mass spectrometry spectrum (MS/MS) information was acquired through an enhanced product ion (EPI) scan. Nine major GTs were identified in tree peony. To quantify the targeted GTs in different tissues of tree peony, we established a multiple reaction monitoring (MRM)-enhanced product ion (EPI)-based pseudo-targeted approach under the information-dependent acquisition (IDA) mode. The quantitative results show that the GT compounds were ubiquitous in tree peony plants with diverse structures. The typical GT PGG was mainly distributed in roots, leaves, and petals. This strategy can also be utilized for metabolite characterization and quantification in other substrates.
没食子单宁（GTs）是一系列具有多种促进健康作用的可水解单宁。在本研究中，开发了一种液相色谱串联质谱（LC-MS/MS）综合策略，用于揭示新兴油料作物中GTs的空间分布模式。根据代表性GT（1,2,3,4,6-五没食子酰基-β-D-葡萄糖，PGG）的裂解行为，选择170 Da的诊断中性丢失（NL）用于GT前体的非靶向筛选。同时，通过增强产物离子（EPI）扫描获得串联质谱（MS/MS）信息。在牡丹中鉴定出9种主要的GTs。为了定量牡丹不同组织中的靶向GTs，我们在信息依赖采集（IDA）模式下建立了一种基于多反应监测（MRM）-增强产物离子（EPI）的伪靶向方法。定量结果表明，GT化合物在结构多样的牡丹植株中普遍存在。典型的GT PGG主要分布在根、叶和花瓣中。该策略也可用于其他基质中代谢物的表征和定量。
