Travaglino Antonio, Raffone Antonio, Saccone Gabriele, Migliorini Sonia, Maruotti Giuseppe Maria, Esposito Gennaro, Mollo Antonio, Martinelli Pasquale, Zullo Fulvio, D'Armiento Maria
Anatomic Pathology Unit, Department of Advanced Biomedical Sciences, School of Medicine, University of Naples Federico II, Naples, Italy.
Department of Neuroscience, Reproductive Sciences and Dentistry, School of Medicine, University of Naples Federico II, Naples, Italy.
Eur J Obstet Gynecol Reprod Biol. 2019 Mar;234:200-206. doi: 10.1016/j.ejogrb.2018.12.039. Epub 2019 Jan 14.
Early-onset preeclampsia is a form of preeclampsia requiring delivery before 34 weeks of gestation. The etiology is unknown, but placental dysfunction appears crucial. We evaluated the immunohistochemical expression of the antiapoptotic protein Bcl-2, the angiogenetic factors VEGF and PlGF, and the epithelial factors HGF, c-Met and STAT3 in placental samples of pregnancies complicated by early-onset preeclampsia.
Placental sections were obtained from 41 women with early-onset preeclampsia (cases) and from 31 uncomplicated pregnancies (controls). A standard haematoxylin and eosin stain was used to assess histological structure. Immunohistochemical expression of Bcl-2, VEGF, PlGF, HGF, c-Met and STAT3 was analyzed.
Mean gestational age was 32 weeks in cases and 39 weeks in controls. Microscopically, sections from women with preeclampsia showed a disorder of villous development as a distal villous hypoplasia with placental undergrowth. The immunoistochemical expression of Bcl-2 (p < 0.0001), VEGF (p = 0.0323), PlGF (p = 0.002), HGF (p < 0.0001), c-Met (p < 0.0001) and STAT3 (p = 0.0004) were significantly lower in placentas of complicated pregnancies compared to uncomplicated ones.
Early-onset preeclampsia is associated with a disorder of villous development. Apoptotic, angiogenetic and epithelial mechanisms are simultaneously impaired and contribute to placental dysfunctions.
早发型子痫前期是子痫前期的一种形式，需要在妊娠34周前分娩。其病因尚不清楚，但胎盘功能障碍似乎至关重要。我们评估了抗凋亡蛋白Bcl-2、血管生成因子VEGF和PlGF以及上皮因子HGF、c-Met和STAT3在早发型子痫前期合并妊娠胎盘样本中的免疫组化表达。
从41例早发型子痫前期妇女（病例组）和31例正常妊娠妇女（对照组）获取胎盘切片。采用标准苏木精-伊红染色评估组织结构。分析Bcl-2、VEGF、PlGF、HGF、c-Met和STAT3的免疫组化表达。
病例组平均孕周为32周，对照组为39周。显微镜下，子痫前期妇女的切片显示绒毛发育紊乱，表现为远端绒毛发育不全和胎盘生长不足。与正常妊娠胎盘相比，复杂妊娠胎盘的Bcl-2（p < 0.0001）、VEGF（p = 0.0323）、PlGF（p = 0.002）、HGF（p < 0.0001）、c-Met（p < 0.0001）和STAT3（p = 0.0004）免疫组化表达显著降低。
早发型子痫前期与绒毛发育紊乱有关。凋亡、血管生成和上皮机制同时受损，导致胎盘功能障碍。
