Liang Junmin, Wan Qiong, Luo Yong, Ma Zhanhong
Department of Plant Pathology, China Agricultural University, Beijing 100193, P. R. China.
Plant Dis. 2013 Apr;97(4):501-509. doi: 10.1094/PDIS-01-12-0072-RE.
Wheat stripe rust, caused by the fungal pathogen Puccinia striiformis f. sp. tritici, is one of the most destructive plant diseases in China. Gansu and Ningxia Provinces are considered to be the key areas for over-summering of this pathogen in China. In this study, 283 P. striiformis f. sp. tritici isolates were collected in these two provinces. Amplified fragment length polymorphism (AFLP) was used to analyze the population genetics and to infer the chance of population exchanges between different geographic locations and seasons. The genotypic diversity of the Gansu population (0.514) was slightly higher than that of the Ningxia population (0.489). The occurrence of frequent population exchanges between these two regions was observed, showing that 40 AFLP genotypes were shared by the populations of the two provinces. Gene flow between these two regions in autumn and spring subpopulations was also detected. The genotype distribution in three populations of Ningxia from opposite sides of the Liupan Mountains revealed possible significant effects of the mountains on limiting gene flow and population exchange. Phylogenetic analysis confirmed the possibility of recombination in some of the studied subpopulations in both provinces.
由真菌病原体条形柄锈菌小麦专化型（Puccinia striiformis f. sp. tritici）引起的小麦条锈病是中国最具毁灭性的植物病害之一。甘肃省和宁夏回族自治区被认为是该病原体在中国的越夏关键区域。在本研究中，从这两个省份采集了283个条形柄锈菌小麦专化型分离株。利用扩增片段长度多态性（AFLP）分析群体遗传学，并推断不同地理位置和季节之间群体交换的可能性。甘肃群体的基因型多样性（0.514）略高于宁夏群体（0.489）。观察到这两个地区之间频繁发生群体交换，表明两个省份的群体共有40种AFLP基因型。还检测到秋季和春季亚群体在这两个地区之间的基因流动。六盘山西侧和东侧宁夏三个群体的基因型分布揭示了山脉对限制基因流动和群体交换可能产生的显著影响。系统发育分析证实了两个省份部分研究亚群体中存在重组的可能性。
