State Key Laboratory of Biology for Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100094, China.
Mycologia. 2010 Jan-Feb;102(1):44-53. doi: 10.3852/08-098.
Puccinia striiformis f.sp. tritici (PST), a basidiomycota responsible for wheat yellow rust, has a strict clonal behavior and a low genetic diversity in European and Australian populations. On the other hand high diversity has been reported in Chinese populations. Moreover it is thought that in China yellow rust epidemics start recurrently from the western highlands where over-summering occurs. To compare PST genetic diversity in this area to the one described in France seven AFLP primer combinations were used to analyze a sample of 160 isolates collected in 2001 in five counties of Gansu Province. The AFLP data revealed 40 polymorphic bands, discriminating 139 AFLP genotypes. Linkage disequilibrium and phylogeographic analyses support the hypothesis of a reproductive mode that is not strictly clonal. In this regard Chinese isolates from Gansu strongly contrast with the European studies using the same markers. Genetic diversity of this 1 y sampling in Gansu is found to be seven times higher than the one observed in France over 20 y and exhibits lower linkage disequilibrium. The effective population size of the French sample was estimated to be 1000 times smaller than the Gansu population. These results support the hypothesis of large population size as well as the occurrence of genetic recombination, while the importance of Gansu as a main over-summering area requires assessment through larger scale studies.
条锈菌（Puccinia striiformis f.sp. tritici，PST）是一种担子菌，可引起小麦条锈病，其在欧洲和澳大利亚种群中表现出严格的无性行为和较低的遗传多样性。另一方面，在中国种群中报道了较高的多样性。此外，人们认为在中国，条锈病的流行是从西部高海拔地区反复发生的，这些地区是越夏的地方。为了比较该地区 PST 的遗传多样性与法国所描述的多样性，使用 7 种 AFLP 引物组合分析了 2001 年在甘肃省五个县采集的 160 个分离物的样本。AFLP 数据显示出 40 个多态性条带，可区分 139 个 AFLP 基因型。连锁不平衡和系统地理学分析支持了一种非严格无性的生殖模式的假说。在这方面，来自甘肃的中国分离物与使用相同标记的欧洲研究强烈对比。在甘肃的 1 年采样中发现的遗传多样性是在法国 20 年观察到的遗传多样性的 7 倍，且表现出较低的连锁不平衡。法国样本的有效种群大小估计比甘肃种群小 1000 倍。这些结果支持了大种群大小以及遗传重组发生的假说，而甘肃作为主要越夏地区的重要性需要通过更大规模的研究来评估。
