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采用顶空固相微萃取-气相色谱-质谱联用(HS-SPME-GC-MS)和顶空固相微萃取-气相色谱-嗅觉检测联用(HS-SPME-GC-O/FID)分析野生食用北欧蘑菇的致香挥发性化合物。

Odor-contributing volatile compounds of wild edible Nordic mushrooms analyzed with HS-SPME-GC-MS and HS-SPME-GC-O/FID.

机构信息

University of Turku, Department of Biochemistry, Food Chemistry and Food Development, FI-20014 Turku, Finland; University of Turku, Functional Foods Forum, FI-20014 Turku, Finland.

University of Turku, Department of Biochemistry, Food Chemistry and Food Development, FI-20014 Turku, Finland.

出版信息

Food Chem. 2019 Jun 15;283:566-578. doi: 10.1016/j.foodchem.2019.01.053. Epub 2019 Jan 17.

Abstract

Although Nordic wild edible mushrooms offer a wide range of different odors their scientific examination has been scarce. The aim of this study was to characterize the aroma compounds of four Finnish wild mushroom species with trained assessors using gas chromatography-olfactometry as well as gas chromatography-mass spectrometry. Headspace volatiles were extracted from sous vide cooked mushroom samples (Boletus edulis, Lactarius camphoratus, Cantharellus cibarius and Craterellus tubaeformis) using solid-phase microextraction. Odor-contributing compounds were measured with two columns of differing polarity using the detection frequency method. Compounds were identified based on reference compounds, linear retention indices, odor descriptions, and mass spectrometry. Both the volatile compound profiles and the aromagrams were distinct with characteristic compounds for each species. The results demonstrate that especially saturated and unsaturated aldehydes and ketones contribute to the odor of the studied wild mushrooms. This thorough comparison also indicates compounds linked to the sensory properties of mushrooms.

摘要

虽然北欧野生食用蘑菇具有广泛的不同气味,但对其进行科学研究却很少。本研究的目的是使用气相色谱-嗅觉测量法和气相色谱-质谱联用仪,通过训练有素的评估人员对四种芬兰野生蘑菇的香气化合物进行特征描述。采用固相微萃取法从真空烹饪蘑菇样品(牛肝菌、乳菇、鸡油菌和喇叭菌)中提取顶空挥发物。使用两种不同极性的色谱柱,采用检测频率法测定有气味的化合物。根据参考化合物、线性保留指数、气味描述和质谱对化合物进行鉴定。挥发性化合物的图谱和香气图谱都具有特征性,每种蘑菇都有其特征化合物。结果表明,特别是饱和和不饱和醛酮对所研究的野生蘑菇的气味有贡献。这种彻底的比较还表明了与蘑菇感官特性相关的化合物。

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