Sesame bran as an unexploited by-product: Effect of enzyme and ultrasound-assisted extraction on the recovery of protein and antioxidant compounds.

Significant amount of bran is discarded from sesame processing plants and yet it is seen as waste or animal feed. This study for the first time designed to recover protein and antioxidant compounds from sesame bran. In this respect, effectiveness of four different techniques i.e. viscozyme L, alcalase, ultrasound and ultrasound-assisted enzymatic extractions were tested and compared with standard alkaline method. RSM was used to investigate the effects of extraction parameters and to determine optimum process conditions. All of the independent parameters (enzyme concentrations, pH, ultrasound power, temperature and time) had significant effects on all of the responses. Alcalase exhibited higher recovery efficiency than viscozyme L. The highest protein yield, total phenolic compound and antioxidant capacities were found in ultrasound-assisted enzymatic extraction at 836 W ultrasound power, 43 °C, 98 min, 9.8 pH value and 1.248 AU/100 g enzyme concentration. SDS-PAGE and SEM analyses were also carried out to compare extraction techniques.