Chinese Academy of Medical Sciences, Peking Union Medical College Hospital, Beijing, China.
Sci Rep. 2019 Feb 5;9(1):1442. doi: 10.1038/s41598-018-37452-3.
The purpose of this study was to explore the potential relationship between intravenous leiomyomatosis (IVL) and uterine myoma (UM) at the molecular level. RNA-sequencing was performed on IVL tumours, UM tumours, and adjacent normal uterine muscle. We compared the gene expression levels between IVL and normal uterine muscle, UM and normal uterine muscle, to identify differentially expressed genes (DEGs). Then we used Gene Ontology Enrichment Analysis to determine the functions of the DEGs and performed specimen cluster analysis. We obtained 98 DEGs between IVL and adjacent normal uterine muscle, and 61 DEGs between UM and adjacent normal uterine muscle. Functional enrichment of both IVL and UM DEGs showed that they are associated with hormone stimulus, extracellular matrix, and cell adhesion. Unsupervised clustering analysis showed that IVL and UM could not be separated completely. Among these dysregulated genes, we found that HOXA13 showed a distinct dysregulated status between IVL and UM. HOXA13 may therefore serves as a biomarker to distinguish IVL and UM. Our results showed that IVL and UM may have similar dysregulated gene networks. They may be closely related, and HOXA13 may serves as a biomarker to distinguish between IVL and UM.
本研究旨在探讨静脉内平滑肌瘤病（IVL）与子宫肌瘤（UM）在分子水平上的潜在关系。对 IVL 肿瘤、UM 肿瘤和相邻正常子宫肌组织进行 RNA 测序。我们比较了 IVL 与正常子宫肌、UM 与正常子宫肌之间的基因表达水平，以鉴定差异表达基因（DEGs）。然后，我们使用基因本体论富集分析来确定 DEGs 的功能，并进行标本聚类分析。我们获得了 98 个 IVL 与相邻正常子宫肌之间的 DEGs 和 61 个 UM 与相邻正常子宫肌之间的 DEGs。IVL 和 UM 的 DEGs 的功能富集表明它们与激素刺激、细胞外基质和细胞黏附有关。无监督聚类分析表明，IVL 和 UM 不能完全分开。在这些失调基因中，我们发现 HOXA13 在 IVL 和 UM 之间表现出明显的失调状态。因此，HOXA13 可以作为区分 IVL 和 UM 的生物标志物。我们的结果表明，IVL 和 UM 可能具有相似的失调基因网络。它们可能密切相关，HOXA13 可以作为区分 IVL 和 UM 的生物标志物。
