• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

基于甘草酸修饰的DSPE-PEG-PEI纳米颗粒的肝靶向联合疗法用于阿霉素和Bcl-2 siRNA的共递送

Liver-Targeted Combination Therapy Basing on Glycyrrhizic Acid-Modified DSPE-PEG-PEI Nanoparticles for Co-delivery of Doxorubicin and Bcl-2 siRNA.

作者信息

Tian Guixiang, Pan Ruiyan, Zhang Bo, Qu Meihua, Lian Bo, Jiang Hong, Gao Zhiqin, Wu Jingliang

机构信息

School of Bioscience and Technology, Weifang Medical University, Weifang, China.

School of Pharmacy, Weifang Medical University, Weifang, China.

出版信息

Front Pharmacol. 2019 Jan 22;10:4. doi: 10.3389/fphar.2019.00004. eCollection 2019.

DOI:10.3389/fphar.2019.00004
PMID:30723405
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6349772/
Abstract

Combination therapy based on nano-sized drug delivery system has been developed as a promising strategy by combining two or more anti-tumor mechanisms. Here, we prepared liver-targeted nanoparticles (GH-DPP) composed of 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-polyethylene glycol-polyetherimide (DSPE-PEG-PEI) with Glycyrrhetinic acid-modified hyaluronic acid (GA-HA) for co-delivery of doxorubicin (DOX) and Bcl-2 siRNA. Particles size, zeta potential and morphology were determined for the drug-loaded GH-DPP nanoparticles (siRNA/DOX/GH-DPP). Cellular uptake and cytotoxicity were analyzed against HepG2 cells. bio-distribution and anti-tumor therapeutic effects of siRNA/DOX/GH-DPP were evaluated in H22-bearing mice. The results showed that siRNA/DOX/GH-DPP nanoparticles were nearly spherical and showed dose-dependent cytotoxicity against HepG2 cells. Compared to Glycyrrhetinic acid-free co-delivery system (siRNA/DOX/DPP) and GH-DPP nanoparticles for delivery of DOX or Bcl-2 siRNA alone, siRNA/DOX/GH-DPP nanoparticles could induce more cellular apoptosis, and showed higher anti-tumor effect. Herein GH-DPP nanoparticles could simultaneously deliver both chemotherapy drugs and siRNA into the tumor region, exhibiting great potential in anti-tumor therapy.

摘要

基于纳米级药物递送系统的联合疗法通过结合两种或更多种抗肿瘤机制已被开发为一种有前景的策略。在此，我们制备了由1,2-二硬脂酰-sn-甘油-3-磷酸乙醇胺-聚乙二醇-聚醚酰亚胺（DSPE-PEG-PEI）与甘草次酸修饰的透明质酸（GA-HA）组成的肝靶向纳米颗粒（GH-DPP），用于共递送阿霉素（DOX）和Bcl-2小干扰RNA（siRNA）。测定了载药的GH-DPP纳米颗粒（siRNA/DOX/GH-DPP）的粒径、ζ电位和形态。分析了其对HepG2细胞的细胞摄取和细胞毒性。在荷H22小鼠中评估了siRNA/DOX/GH-DPP的生物分布和抗肿瘤治疗效果。结果表明，siRNA/DOX/GH-DPP纳米颗粒近乎球形，对HepG2细胞表现出剂量依赖性细胞毒性。与不含甘草次酸的共递送系统（siRNA/DOX/DPP）以及单独用于递送DOX或Bcl-2 siRNA的GH-DPP纳米颗粒相比，siRNA/DOX/GH-DPP纳米颗粒可诱导更多的细胞凋亡，并显示出更高的抗肿瘤效果。在此，GH-DPP纳米颗粒可同时将化疗药物和siRNA递送至肿瘤区域，在抗肿瘤治疗中展现出巨大潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d65a/6349772/f442a2f8c49c/fphar-10-00004-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d65a/6349772/e7e63914f1ce/fphar-10-00004-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d65a/6349772/5b6e9cf9e8cd/fphar-10-00004-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d65a/6349772/6a74c00610bb/fphar-10-00004-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d65a/6349772/c7689f3b3afb/fphar-10-00004-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d65a/6349772/28326abc8ba6/fphar-10-00004-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d65a/6349772/46233e75668a/fphar-10-00004-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d65a/6349772/4f05ee3e4cab/fphar-10-00004-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d65a/6349772/f442a2f8c49c/fphar-10-00004-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d65a/6349772/e7e63914f1ce/fphar-10-00004-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d65a/6349772/5b6e9cf9e8cd/fphar-10-00004-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d65a/6349772/6a74c00610bb/fphar-10-00004-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d65a/6349772/c7689f3b3afb/fphar-10-00004-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d65a/6349772/28326abc8ba6/fphar-10-00004-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d65a/6349772/46233e75668a/fphar-10-00004-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d65a/6349772/4f05ee3e4cab/fphar-10-00004-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d65a/6349772/f442a2f8c49c/fphar-10-00004-g008.jpg

相似文献

1
Liver-Targeted Combination Therapy Basing on Glycyrrhizic Acid-Modified DSPE-PEG-PEI Nanoparticles for Co-delivery of Doxorubicin and Bcl-2 siRNA.基于甘草酸修饰的DSPE-PEG-PEI纳米颗粒的肝靶向联合疗法用于阿霉素和Bcl-2 siRNA的共递送
Front Pharmacol. 2019 Jan 22;10:4. doi: 10.3389/fphar.2019.00004. eCollection 2019.
2
Lipid-polymer hybrid nanoparticles as a new generation therapeutic delivery platform: a review.脂质-聚合物杂化纳米颗粒作为新一代治疗性递送平台：综述
Eur J Pharm Biopharm. 2013 Nov;85(3 Pt A):427-43. doi: 10.1016/j.ejpb.2013.07.002. Epub 2013 Jul 17.
3
EGF-modified mPEG-PLGA-PLL nanoparticle for delivering doxorubicin combined with Bcl-2 siRNA as a potential treatment strategy for lung cancer.表皮生长因子修饰的甲氧基聚乙二醇-聚乳酸-羟基乙酸共聚物-聚赖氨酸纳米粒用于递送阿霉素并联合Bcl-2小干扰RNA作为肺癌的一种潜在治疗策略。
Drug Deliv. 2016 Oct;23(8):2936-2945. doi: 10.3109/10717544.2015.1126769. Epub 2016 Jan 7.
4
Enhanced delivery of doxorubicin to the liver through self-assembled nanoparticles formed via conjugation of glycyrrhetinic acid to the hydroxyl group of hyaluronic acid.通过将甘草次酸的羟基与透明质酸缀合形成自组装纳米粒子，增强了阿霉素向肝脏的递送。
Carbohydr Polym. 2018 Sep 1;195:170-179. doi: 10.1016/j.carbpol.2018.04.052. Epub 2018 Apr 13.
5
Glycyrrhetinic acid-modified chitosan/poly(ethylene glycol) nanoparticles for liver-targeted delivery.甘草次酸修饰的壳聚糖/聚乙二醇纳米粒用于肝靶向递药。
Biomaterials. 2010 Jun;31(17):4748-56. doi: 10.1016/j.biomaterials.2010.02.042. Epub 2010 Mar 19.
6
Glycyrrhetinic acid-conjugated polymeric prodrug micelles co-delivered with doxorubicin as combination therapy treatment for liver cancer.甘草次酸接枝聚合物前药胶束共载多柔比星用于肝癌联合治疗。
Colloids Surf B Biointerfaces. 2019 Mar 1;175:106-115. doi: 10.1016/j.colsurfb.2018.11.082. Epub 2018 Nov 30.
7
Pulmonary Codelivery of Doxorubicin and siRNA by pH-Sensitive Nanoparticles for Therapy of Metastatic Lung Cancer.pH 敏感型纳米粒肺部共递药系统治疗转移性肺癌：阿霉素与 siRNA 的联合递送
Small. 2015 Sep 9;11(34):4321-33. doi: 10.1002/smll.201501034. Epub 2015 Jul 1.
8
Doxorubicin-loaded glycyrrhetinic acid modified recombinant human serum albumin nanoparticles for targeting liver tumor chemotherapy.负载阿霉素的甘草次酸修饰重组人血清白蛋白纳米粒用于靶向性肝癌化疗
Mol Pharm. 2015 Mar 2;12(3):675-83. doi: 10.1021/mp500394v. Epub 2015 Feb 5.
9
Investigation of Dual-Loaded Doxorubicin and Sorafenib Liposomes Co-Modified with Glycyrrhetinic Acid and Cell-Penetrating Peptide TAT.甘草次酸和细胞穿透肽TAT共修饰的双载阿霉素和索拉非尼脂质体的研究
Curr Drug Deliv. 2024 Sep 3. doi: 10.2174/0115672018320991240903060726.
10
A self-assembled polyjuglanin nanoparticle loaded with doxorubicin and anti-Kras siRNA for attenuating multidrug resistance in human lung cancer.一种负载阿霉素和抗Kras小干扰RNA的自组装聚胡桃素纳米颗粒，用于减轻人肺癌中的多药耐药性。
Biochem Biophys Res Commun. 2017 Dec 2;493(4):1430-1437. doi: 10.1016/j.bbrc.2017.09.132. Epub 2017 Sep 25.

引用本文的文献

1
Medicinal plants: nutritional, immunological and therapeutic role in treating cancer-related malnutrition: a comprehensive review.药用植物：在治疗癌症相关营养不良中的营养、免疫及治疗作用：一项综述
Cancer Cell Int. 2025 Jul 16;25(1):266. doi: 10.1186/s12935-025-03720-2.
2
Improved Photodynamic Therapy of Hepatocellular Carcinoma via Surface-Modified Protein Nanoparticles.通过表面修饰的蛋白质纳米颗粒改善肝细胞癌的光动力疗法
Pharmaceutics. 2025 Mar 14;17(3):370. doi: 10.3390/pharmaceutics17030370.
3
Complexed hyaluronic acid-based nanoparticles in cancer therapy and diagnosis: Research trends by natural language processing.

本文引用的文献

1
Polymer-drug conjugate therapeutics: advances, insights and prospects.聚合物-药物偶联物治疗学：进展、见解与展望。
Nat Rev Drug Discov. 2019 Apr;18(4):273-294. doi: 10.1038/s41573-018-0005-0.
2
Magnetic Nanomaterials for Advanced Regenerative Medicine: The Promise and Challenges.用于高级再生医学的磁性纳米材料：前景与挑战。
Adv Mater. 2019 Nov;31(45):e1804922. doi: 10.1002/adma.201804922. Epub 2018 Dec 4.
3
Glycyrrhetinic Acid Functionalized Nanoparticles for Drug Delivery to Liver Cancer.甘草次酸功能化纳米粒子用于肝癌药物递送。
基于复合透明质酸的纳米颗粒在癌症治疗与诊断中的应用：通过自然语言处理的研究趋势
Heliyon. 2024 Dec 18;11(1):e41246. doi: 10.1016/j.heliyon.2024.e41246. eCollection 2025 Jan 15.
4
Multi-functional near-infrared fluorescent polymer dot-siRNA for gene expression regulation.用于基因表达调控的多功能近红外荧光聚合物点-小干扰RNA
J Mater Chem B. 2025 Feb 5;13(6):2124-2139. doi: 10.1039/d4tb01954g.
5
3D cryo-printed hierarchical porous scaffolds provide immobilization of surface-functionalized sleep-inspired small extracellular vesicles: synergistic therapeutic strategies for vascularized bone regeneration based on macrophage phenotype modulation and angiogenesis-osteogenesis coupling.3D 冷冻打印分层多孔支架可固定表面功能化的睡眠启发型小细胞外囊泡：基于巨噬细胞表型调节和血管生成-骨生成耦合的血管化骨再生协同治疗策略。
J Nanobiotechnology. 2024 Dec 19;22(1):764. doi: 10.1186/s12951-024-02977-5.
6
The advancement of siRNA-based nanomedicine for tumor therapy.基于 siRNA 的纳米医学在肿瘤治疗中的进展。
Nanomedicine (Lond). 2024;19(21-22):1841-1862. doi: 10.1080/17435889.2024.2377062. Epub 2024 Aug 15.
7
Advances in active targeting of ligand-directed polymeric nanomicelles exploiting overexpressed cellular receptors for precise nanomedicine.利用过表达的细胞受体进行精确纳米医学的配体导向聚合物纳米胶束主动靶向研究进展。
RSC Adv. 2024 Jul 26;14(32):23520-23542. doi: 10.1039/d4ra04069d. eCollection 2024 Jul 19.
8
Advances in the Study of Bioactive Nanoparticles for the Treatment of HCC and Its Postoperative Residual Cancer.用于 HCC 及其术后残余癌治疗的生物活性纳米粒子的研究进展。
Int J Nanomedicine. 2023 May 22;18:2721-2735. doi: 10.2147/IJN.S399146. eCollection 2023.
9
Formulation and Characterization of FeO@PEG Nanoparticles Loaded Sorafenib; Molecular Studies and Evaluation of Cytotoxicity in Liver Cancer Cell Lines.负载索拉非尼的FeO@PEG纳米颗粒的制备与表征；肝癌细胞系的分子研究及细胞毒性评估
Polymers (Basel). 2023 Feb 16;15(4):971. doi: 10.3390/polym15040971.
10
Potential of siRNA in COVID-19 therapy: Emphasis on design and nanoparticles based delivery.siRNA在COVID-19治疗中的潜力：重点在于设计和基于纳米颗粒的递送。
Front Bioeng Biotechnol. 2023 Feb 6;11:1112755. doi: 10.3389/fbioe.2023.1112755. eCollection 2023.
J Biomed Nanotechnol. 2018 Nov 1;14(11):1837-1852. doi: 10.1166/jbn.2018.2638.
4
Advantages and Limitations of Current Techniques for Analyzing the Biodistribution of Nanoparticles.当前纳米颗粒生物分布分析技术的优势与局限
Front Pharmacol. 2018 Aug 14;9:802. doi: 10.3389/fphar.2018.00802. eCollection 2018.
5
Dual-Responsive Core Crosslinking Glycopolymer-Drug Conjugates Nanoparticles for Precise Hepatocarcinoma Therapy.用于精准肝癌治疗的双响应性核交联糖聚合物-药物缀合物纳米颗粒
Front Pharmacol. 2018 Jul 17;9:663. doi: 10.3389/fphar.2018.00663. eCollection 2018.
6
Co-Delivery of Doxorubicin and Anti-BCL-2 siRNA by pH-Responsive Polymeric Vector to Overcome Drug Resistance in In Vitro and In Vivo HepG2 Hepatoma Model.通过 pH 响应性聚合物载体共递送阿霉素和抗 BCL-2 siRNA 以克服体外和体内 HepG2 肝癌模型中的耐药性。
Biomacromolecules. 2018 Jun 11;19(6):2248-2256. doi: 10.1021/acs.biomac.8b00272. Epub 2018 May 3.
7
Advances in microfluidics for lipid nanoparticles and extracellular vesicles and applications in drug delivery systems.微流控技术在脂质纳米粒和细胞外囊泡方面的进展及其在药物传递系统中的应用。
Adv Drug Deliv Rev. 2018 Mar 15;128:84-100. doi: 10.1016/j.addr.2018.03.008. Epub 2018 Mar 19.
8
Acid-sensitive polymeric vector targeting to hepatocarcinoma cells via glycyrrhetinic acid receptor-mediated endocytosis.基于甘草次酸受体介导的内吞作用的靶向肝癌细胞的酸敏感型聚合物载体。
Mater Sci Eng C Mater Biol Appl. 2018 Jun 1;87:32-40. doi: 10.1016/j.msec.2018.02.013. Epub 2018 Feb 21.
9
Quantitative self-assembly prediction yields targeted nanomedicines.定量自组装预测可得到靶向纳米药物。
Nat Mater. 2018 Apr;17(4):361-368. doi: 10.1038/s41563-017-0007-z. Epub 2018 Feb 5.
10
Combinational siRNA delivery using hyaluronic acid modified amphiphilic polyplexes against cell cycle and phosphatase proteins to inhibit growth and migration of triple-negative breast cancer cells.使用透明质酸修饰的两亲性聚电解质复合物递送来抑制三阴性乳腺癌细胞的生长和迁移：针对细胞周期和磷酸酶蛋白的组合 siRNA 递送。
Acta Biomater. 2018 Jan 15;66:294-309. doi: 10.1016/j.actbio.2017.11.036. Epub 2017 Nov 26.