Sf Albaayit, Maharjan Rukesh
Pak J Biol Sci. 2018 Jan;21(9):475-479. doi: 10.3923/pjbs.2018.475.479.
Zerumbone has been reported to exert anti-inflammatory, anti-ulcer and anti-hyperglycemic effects but the specific mechanism through which zerumbone exerts its anti-inflammatory action through inhibiting reactive oxygen species was not well studied. Hence, this paper studied the zerumbone capacity to inhibit intracellular and extracellular Reactive Oxygen Species (ROS) produced by whole blood cell, polymorphoneutrophil (PMNs) and macrophage cells due to the zymogen and phorbolmyristerate acetate (PMA) oxidant effect.
Zymogen and PMA based chemiluminescence assay were used to determine the immunomodulatory effect of zerumbone at concentrations (100, 10 and 1 μg mL-1) toward production of Reactive Oxygen Species (ROS) from whole blood, PMNs and macrophage.
Zerumbone significantly inhibited intracellular and extracellular ROS production by the zymosan/PMA-activated phagocyte cells with IC50 values of (16.3±0.1, 23.7±0.1 and 4.97±0.1 μg mL-1) against whole blood, PMNs and macrophage respectively.
The anti-inflammatory activity of zerumbone was so much significant that even strong oxidant (zymogen and PMA) were not able to produce reactive oxygen species when incubated together in phagocytic cells, thus suppress production of ROS. Therefore, it is highly used in herbal medicine as a potent immunomodulatory therapy in various inflammation associated diseases.
据报道，姜黄烯具有抗炎、抗溃疡和降血糖作用，但姜黄烯通过抑制活性氧发挥抗炎作用的具体机制尚未得到充分研究。因此，本文研究了姜黄烯抑制全血细胞、多形核中性粒细胞（PMN）和巨噬细胞因酶原和佛波酯（PMA）氧化作用产生的细胞内和细胞外活性氧（ROS）的能力。
采用基于酶原和PMA的化学发光测定法，测定姜黄烯在浓度为100、10和1μg/mL时对全血、PMN和巨噬细胞产生活性氧（ROS）的免疫调节作用。
姜黄烯显著抑制酵母聚糖/PMA激活的吞噬细胞产生细胞内和细胞外ROS，对全血、PMN和巨噬细胞的IC50值分别为（16.3±0.1、23.7±0.1和4.97±0.1μg/mL）。
姜黄烯的抗炎活性非常显著，以至于在吞噬细胞中与强氧化剂（酶原和PMA）一起孵育时，它们也无法产生活性氧，从而抑制了ROS的产生。因此，它在草药中被广泛用作治疗各种炎症相关疾病的有效免疫调节疗法。
