姜黄根茎的标准化乙醇提取物、精油和姜烯酮抑制人中性粒细胞的吞噬活性。
Standardized ethanol extract, essential oil and zerumbone of Zingiber zerumbet rhizome suppress phagocytic activity of human neutrophils.
机构信息
Drug and Herbal Research Centre, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, 50300, Kuala Lumpur, Malaysia.
School of Pharmacy, Faculty of Health and Medical Sciences, Taylor's University, Lakeside Campus, 47500, Subang Jaya, Selangor, Malaysia.
出版信息
BMC Complement Altern Med. 2019 Nov 21;19(1):331. doi: 10.1186/s12906-019-2748-5.
BACKGROUND
Zingiber zerumbet rhizome and its bioactive metabolites have previously been reported to exhibit innumerable pharmacological properties particularly anti-inflammatory activities. In the present study, the 80% ethanol extract, essential oil and zerumbone of Z. zerumbet rhizomes were explored for their in vitro immunosuppressive properties on chemotaxis, CD11b/CD18 expression, phagocytosis and chemiluminescence of isolated human polymorphonuclear neutrophils (PMNs).
METHODS
The extract was analyzed quantitatively by performing a validated reversed phase high performance liquid chromatography (RP-HPLC). Zerumbone was isolated by chromatographic technique while the essential oil was acquired through hydro-distillation of the rhizomes and further analyzed by gas chromatography (GC) and GC-MS. Chemotaxis assay was assessed by using a 24-well cell migration assay kit, while CD18 integrin expression and phagocytic engulfment were measured using flow cytometry. The reactive oxygen species (ROS) production was evaluated by applying lucigenin- and luminol-enhanced chemiluminescence assays.
RESULTS
Zerumbone was found to be the most abundant compound in the extract (242.73 mg/g) and the oil (58.44%). Among the samples tested, the oil revealed the highest inhibition on cell migration with an IC value of 3.24 μg/mL. The extract, oil and zerumbone showed moderate inhibition of CD18 integrin expression in a dose-dependent trend. Z. zerumbet extract showed the highest inhibitory effect on phagocytic engulfment with percentage of phagocytizing cells of 55.43% for PMN. Zerumbone exhibited strong inhibitory activity on oxidative burst of zymosan- and PMA-stimulated neutrophils. Zerumbone remarkably inhibited extracellular ROS production in PMNs with an IC value of 17.36 μM which was comparable to that of aspirin.
CONCLUSION
The strong inhibition on the phagocytosis of neutrophils by Z. zerumbet extract and its essential oil might be due the presence of its chemical components particularly zerumbone which was capable of impeding phagocytosis at different stages.
背景
姜黄属植物莪术的根茎及其生物活性代谢产物先前已被报道具有无数的药理特性，特别是抗炎活性。在本研究中，我们研究了莪术根茎的 80%乙醇提取物、精油和莪术醇的体外免疫抑制特性，包括对分离的人多形核中性粒细胞（PMN）的趋化性、CD11b/CD18 表达、吞噬作用和化学发光的影响。
方法
通过执行经验证的反相高效液相色谱法（RP-HPLC）对提取物进行定量分析。通过色谱技术分离出莪术醇，通过根茎的水蒸馏获得精油，并进一步通过气相色谱（GC）和 GC-MS 进行分析。趋化性测定通过使用 24 孔细胞迁移测定试剂盒进行评估，而 CD18 整合素表达和吞噬作用通过流式细胞术进行测量。通过应用荧光素和鲁米诺增强化学发光测定法评估活性氧（ROS）的产生。
结果
发现莪术醇是提取物（242.73mg/g）和油（58.44%）中含量最丰富的化合物。在所测试的样品中，油显示出对细胞迁移的最高抑制作用，IC 值为 3.24μg/mL。提取物、油和莪术醇表现出对 CD18 整合素表达的适度抑制作用，呈剂量依赖性趋势。莪术属提取物对PMN 的吞噬作用表现出最高的抑制作用，吞噬细胞的百分比为 55.43%。莪术醇对酵母聚糖和 PMA 刺激的中性粒细胞的氧化爆发表现出强烈的抑制活性。莪术醇显著抑制 PMN 中细胞外 ROS 的产生，IC 值为 17.36μM，与阿司匹林相当。
结论
莪术属提取物及其精油对中性粒细胞吞噬作用的强烈抑制作用可能是由于其化学成分，特别是能够在不同阶段阻碍吞噬作用的莪术醇的存在。
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