Centre for Chemistry and Biotechnology, Deakin University, Waurn Ponds, VIC 3217, Australia; NSW Department of Primary Industries, Yanco Agricultural Institute, Yanco, NSW 2703, Australia.
Infrared Microspectroscopy (IRM) Beamline, Australian Synchrotron, Clayton, VIC 3168, Australia.
Food Chem. 2019 Mar 1;275:457-466. doi: 10.1016/j.foodchem.2018.09.043. Epub 2018 Sep 8.
In this study, chia seed oil (CSO) microcapsules were produced using three types of shell materials, including chia seed protein (CPI), chia seed gum (CSG) and CPI-CSG complex coacervates. Synchrotron-Fourier transform infrared (S-FTIR) microspectroscopy was used to investigate the effect of shell materials on the distribution of CSO both on the surface and in the interior of the solid microcapsules. S-FTIR measurements were carried out in macroscopic attenuated total reflection (macro ATR) and transmission modes, to determine the surface lipid and the encapsulated lipid fractions, respectively. The amounts of lipid and protein distributed on the surface and in the interior of the microcapsules were compared based on the average spectra extracted from S-FTIR chemical images obtained from each type of the microcapsules. The unsaturated fatty acids (UFAs) to total oil ratios in all the three types of the microcapsules were closely similar to the original non-processed CSO, suggesting an effective encapsulation and thereby shielding protection of UFAs from oxidative damage during microencapsulation process. The type of the shell materials was found to affect the distribution of CSO on the surface and within the microcapsules. The complex coacervation based microcapsules had a significantly lower oil content (∼2% w/w) on the surface compared to those observed for the other two types of microcapsules (>5%, w/w).
在这项研究中，使用三种壳材料（奇亚籽蛋白（CPI）、奇亚籽胶（CSG）和 CPI-CSG 复合凝聚物）制备了奇亚籽油（CSO）微胶囊。同步辐射傅里叶变换红外（S-FTIR）微光谱用于研究壳材料对固体微胶囊表面和内部 CSO 分布的影响。S-FTIR 测量分别在宏观衰减全反射（macro ATR）和透射模式下进行，以确定表面脂质和包封脂质分数。基于从每种微胶囊获得的 S-FTIR 化学图像提取的平均光谱，比较了脂质和蛋白质在微胶囊表面和内部的分布量。所有三种类型的微胶囊中不饱和脂肪酸（UFAs）与总油的比例与原始未经处理的 CSO 非常相似，这表明在微胶囊化过程中 UFAs 得到了有效的包封和屏蔽保护，从而避免了氧化损伤。发现壳材料的类型影响 CSO 在表面和微胶囊内部的分布。与其他两种类型的微胶囊（>5%，w/w）相比，基于复合凝聚的微胶囊在表面的油含量（约 2%w/w）显著较低。
