2017年至2018年，巴西出现一种导致急性出血性结膜炎的柯萨奇病毒A24变种。
Re-emergence of a coxsackievirus A24 variant causing acute hemorrhagic conjunctivitis in Brazil from 2017 to 2018.
作者信息
Sousa Ivanildo P, Burlandy Fernanda M, Ferreira James L, Alves Jainara C S, Sousa-Júnior Edivaldo C, Tavares Fernando N, da Silva Edson E
机构信息
Laboratório de Enterovírus, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil.
Laboratório de Referência Regional em Enteroviroses, Seção de Virologia, Instituto Evandro Chagas, Rodovia BR 316-KM 07, S/N-Bairro Levilândia, Ananindeua, PA, 67030000, Brazil.
出版信息
Arch Virol. 2019 Apr;164(4):1181-1185. doi: 10.1007/s00705-019-04157-5.
A large outbreak (over 200,000 cases) of acute hemorrhagic conjunctivitis (AHC) took place in Brazil during the summer of 2017/2018, seven years after a nationwide epidemic, which occurred in 2011. To identify the etiological agent, 80 conjunctival swabs from patients with a clinical presentation suggestive of AHC were analyzed at the national enterovirus laboratory. Real-time RT-PCR for human enteroviruses was performed, and enterovirus RNA was detected in 91.25% (73/80) of the specimens. Twenty-nine swab fluids were used to inoculate cell cultures (RD and Hep2C), and 72.4% (21/29) yielded a cytopathic effect. Genotype IV coxsackievirus A24v (CV-A24v) was identified as the causative agent of the outbreak. Phylogenetic analysis based on the VP1 gene revealed that Brazilian isolates were genetically related to strains that caused an outbreak in French Guiana in 2017. Our results show the re-emergence of CV-A24v causing AHC outbreaks in Brazil between the end of 2017 and the beginning of 2018.
2017/2018年夏季，在2011年全国疫情爆发七年后，巴西发生了大规模急性出血性结膜炎（AHC）疫情（超过20万例）。为确定病原体，国家肠道病毒实验室对80份临床表现提示为AHC的患者结膜拭子进行了分析。进行了人肠道病毒的实时逆转录聚合酶链反应（RT-PCR），在91.25%（73/80）的标本中检测到肠道病毒RNA。用29份拭子液接种细胞培养物（RD和Hep2C），72.4%（21/29）产生了细胞病变效应。IV型柯萨奇病毒A24变种（CV-A24v）被确定为此次疫情的病原体。基于VP1基因的系统发育分析表明，巴西分离株与2017年在法属圭亚那引起疫情的毒株在基因上相关。我们的结果显示，2017年底至2018年初，CV-A24v在巴西再度出现并导致AHC疫情爆发。
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