Guangdong Key Laboratory of Integrated Agro-environmental Pollution Control and Management, Guangdong Institute of Eco-Environmental Science and Technology, 808 Tianyuan Road, Guangzhou, 510650, Guangdong, China.
College of Environmental Science and Engineering, Textile Pollution Controlling Engineering Center of Ministry of Environmental Protection, Donghua University, Shanghai, 201620, China.
Environ Sci Pollut Res Int. 2019 Apr;26(10):9585-9598. doi: 10.1007/s11356-019-04336-6. Epub 2019 Feb 6.
Acid mine discharge (AMD) has been demonstrated to have significant impacts on microbial community composition in the surrounding soil environment. However, their effect on adjacent soil has not been extensively studied. In this study, microbial community composition of 20 AMD-contaminated soil samples collected from an abandoned coal mine along an AMD creek was characterized using high-throughput sequencing. All samples were characterized as extremely low in pH (< 3) and relatively enriched in HCl-extractable Fe species. The dominant phylotypes were belonging to genera Ochrobactrum, Acidiphilium, Staphylococcus, Brevibacterium, and Corynebacterium. Canonical correspondence analysis results revealed that the HCl-extractable Fe(III) had a strong impact on the soil microbial assemblage. Co-occurrence network analysis revealed that Aquicella, Acidobacteriaceae, Ochrobactrum, Enhydrobacter, Sphingomonas, and Legionellales were actively correlated with other taxa. As expected, most of the abundant taxa have been reported as acidophilic Fe-metabolizing bacteria. Hence, a co-occurring sub-network and a phylogenetic tree related to microbial taxa responsible for Fe metabolism were constructed and described. The biotic interaction showed that Dechloromonas exhibited densely connections with Fe(III)-reducing bacteria of Comamonas, Burkholderia, Shewanella, Stenotrophomonas, Acidithiobacillus, and Pseudomonas. These results demonstrated that Fe-metabolizing bacteria could have an important role in the Fe biogeochemical cycling.
酸性矿山排水（AMD）已被证明对周围土壤环境中的微生物群落组成有重大影响。然而，它们对相邻土壤的影响尚未得到广泛研究。在这项研究中，使用高通量测序对从 AMD 溪流沿岸废弃煤矿采集的 20 个 AMD 污染土壤样本的微生物群落组成进行了表征。所有样本的 pH 值（<3）极低，HCl 可提取铁物种相对丰富。优势菌群属于 Ochrobactrum、Acidiphilium、Staphylococcus、Brevibacterium 和 Corynebacterium 属。典范对应分析结果表明，HCl 可提取的 Fe(III) 对土壤微生物组合有很强的影响。共生网络分析表明，Aquicella、 Acidobacteriaceae、Ochrobactrum、 Enhydrobacter、Sphingomonas 和 Legionellales 与其他分类群呈积极相关。不出所料，大多数丰度较高的分类群已被报道为嗜酸铁代谢细菌。因此，构建并描述了与铁代谢相关的微生物分类群的共生子网络和系统发育树。生物相互作用表明，Dechloromonas 与 Comamonas、Burkholderia、Shewanella、 Stenotrophomonas、Acidithiobacillus 和 Pseudomonas 的 Fe(III) 还原菌之间存在密集的连接。这些结果表明，铁代谢细菌在铁的生物地球化学循环中可能具有重要作用。
