Li Yang, Wu Min, Zhang Haiqin, Jiang Binbin, Bao Yixiang, Li Jie, Li Jingfeng, Li Peng, Yan Xinyue, Qin Tianqi
State Key Laboratory of Water Resource Protection and Utilization in Coal Mining, National Institute of Low Carbon and Clean Energy, CHN Energy, Beijing 102211, China.
State Key Laboratory of Mining Response and Disaster Prevention and Control in Deep Coal Mines, Anhui University of Science and Technology, Huainan 232001, China.
ACS Omega. 2024 Dec 13;9(51):50786-50795. doi: 10.1021/acsomega.4c09348. eCollection 2024 Dec 24.
Underground reservoirs were used to purify water through filtration, adsorption, and biodegradation methods in coal mines. However, their effects on the composition and metabolism of microbial communities in mine water remain unknown. In this study, influent and effluent samples from underground reservoirs in seven coal mining areas were analyzed to compare their microbial community compositions and metabolites. The results indicate that the underground reservoirs can significantly reduce the chemical oxygen demand (COD) levels of mine water (values range from 7.79 to 8.50 for influent and 7.40 to 8.50 for effluent) and regulate water chemistry characteristics such as pH (values range from 7-1980 for influent and 5-20 for effluent). Since COD reflects the quantity of reducing substances in water, while total dissolved solids (TDS) indicates the total amount of dissolved solids, both parameters directly influence the physicochemical properties of water. This, in turn, affects microbial growth and metabolic pathways. Consequently, changes in these factors can lead to variations in microbial community composition as well as decreases in the Chao1 and Shannon indices. Underground reservoirs decreased the relative abundance of phyla and , and the decreased microbial groups were mainly belonging to phylum . In addition, higher concentrations of metabolites, including lipids and lipid-like molecules, mixed metal/nonmetal compounds, hydrocarbon derivatives, nucleosides, nucleotides, and analogues were detected in the effluent samples, and most of them were related to taurine and hypotaurine metabolism, selenocompound metabolism, glyoxylate and dicarboxylate metabolism, riboflavin metabolism, and the citrate cycle. In summary, this study provided theoretical and experimental support for the evolution mechanism of mine water quality in coal mine underground reservoirs.
煤矿中的地下水库曾被用于通过过滤、吸附和生物降解方法净化水。然而，它们对矿井水中微生物群落组成和代谢的影响仍不清楚。在本研究中，分析了七个煤矿区地下水库的进水和出水样本，以比较它们的微生物群落组成和代谢产物。结果表明，地下水库可以显著降低矿井水的化学需氧量（COD）水平（进水值范围为7.79至8.50，出水值范围为7.40至8.50），并调节水化学特征，如pH值（进水值范围为7 - 1980，出水值范围为5 - 20）。由于COD反映水中还原物质的量，而总溶解固体（TDS）表示溶解固体的总量，这两个参数直接影响水的物理化学性质。反过来，这又影响微生物的生长和代谢途径。因此，这些因素的变化会导致微生物群落组成的变化以及Chao1和Shannon指数的下降。地下水库降低了门 和 的相对丰度，减少的微生物类群主要属于门 。此外，在出水样本中检测到较高浓度的代谢产物，包括脂质和类脂分子、混合金属/非金属化合物、烃衍生物、核苷、核苷酸及其类似物，其中大多数与牛磺酸和亚牛磺酸代谢、硒化合物代谢、乙醛酸和二羧酸代谢、核黄素代谢以及柠檬酸循环有关。总之，本研究为煤矿地下水库中矿井水水质的演化机制提供了理论和实验支持。
