Dybicz Monika, Borkowski Piotr K., Padzik Marcin, Baltaza Wanda, Chomicz Lidia
Department of General Biology and Parasitology, Medical University of Warsaw, ul. Chałubinskiego 5, 02-004 Warsaw, Poland
Department of Infectious Diseases, Tropical and Hepatology, Medical University of Warsaw, Wolska 37, 01-201 Warsaw, Poland
Ann Parasitol. 2018;64(4):339-342. doi: 10.17420/ap6404.169.
In this study five cases of suspected alveolar echinococcosis from Poland in which surgical treatment was needed, previously diagnosed by means of imaging and serological techniques, were analyzed in terms to identify the causative agent. Samples of the parasite tissues taken perioperatively from the liver lesions were used for the histopathological and molecular examinations. The sequences of all isolates were identical to Echinococcus multilocularis; all nad1 sequences have been deposited in GenBank The histopathological examination revealed Passpositive fragments of laminated layers typical for E. multilocularis metacestode. Obtained results confirm that the use of imaging techniques only may be insufficient to diagnose alveococcosis thus the recognition of the zoonosis should base on several procedures; especially valuable are highly sensitive and specific molecular methods.
在本研究中，对来自波兰的5例疑似肺泡型包虫病病例进行了分析，以确定病原体。这些病例此前已通过影像学和血清学技术诊断，均需要进行手术治疗。术中从肝脏病变部位采集的寄生虫组织样本用于组织病理学和分子检查。所有分离株的序列均与多房棘球绦虫相同；所有nad1序列均已存入GenBank。组织病理学检查显示，有典型的多房棘球绦虫成虫层状结构的PAS阳性片段。所得结果证实，仅使用影像学技术可能不足以诊断肺泡型包虫病，因此，对这种人畜共患病的诊断应基于多种检查方法；高度敏感和特异的分子方法尤其有价值。
